| Pesticide multiresidue analysis in cereal grains using modified QuEChERS method combined with automated direct sample introduction GC-TOFMS and UPLC-MS/MS techniques. | |
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MedLine Citation:
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PMID: 20028018 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The QuEChERS (quick, easy, cheap, effective, rugged, and safe) sample preparation method was modified to accommodate various cereal grain matrices (corn, oat, rice, and wheat) and provide good analytical results (recoveries in the range of 70-120% and RSDs <20%) for the majority of the target pesticides (about 180 analytes). The method consists of a 1 h shaking of a milled sample (2.5-5 g) in 20 mL of 1:1 (v/v) water/acetonitrile (or 25 mL of 1.5:1 water/acetonitrile in the case of rice) to provide simultaneous matrix swelling and analyte extraction. Then, a MgSO(4)/NaCl salt mixture (4:1, w/w) is added to the extract to induce phase separation and force the pesticides into the upper acetonitrile layer, a 1 mL aliquot of which is subsequently cleaned up using dispersive solid phase extraction with 150 mg of PSA, 50 mg of C(18), and 150 mg of MgSO(4). GC-amenable pesticides were analyzed using gas chromatography combined with time-of-flight mass spectrometry (GC-TOFMS) and the automated direct sample introduction technique for a large volume injection of the extracts. Ultraperformance liquid chromatography coupled to triple-quadrupole tandem mass spectrometry (UPLC-MS/MS) was employed for the analysis of LC-amenable pesticides. This method was implemented in a routine laboratory, providing about 3-fold increased sample throughput, 40-50% reduction in the cost of disposable materials and in the operation costs, 1:100 solvent waste reduction, and increased scope of the analysis versus the traditional approach based on the Luke method. |
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Authors:
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Katerina Mastovska; Kelly J Dorweiler; Steven J Lehotay; Jennifer S Wegscheid; Kelli A Szpylka |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of agricultural and food chemistry Volume: 58 ISSN: 1520-5118 ISO Abbreviation: J. Agric. Food Chem. Publication Date: 2010 May |
Date Detail:
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Created Date: 2010-05-20 Completed Date: 2010-10-18 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0374755 Medline TA: J Agric Food Chem Country: United States |
Other Details:
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Languages: eng Pagination: 5959-72 Citation Subset: IM |
Affiliation:
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Agricultural Research Service, US Department of Agriculture, Eastern Regional Research Center, 600 East Mermaid Lane, Wyndmoor, Pennsylvania 19038, USA. kmastovska@yahoo.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Autoanalysis Cereals / chemistry* Chemical Fractionation / methods* Chromatography, Liquid / methods* Food Contamination / analysis Gas Chromatography-Mass Spectrometry / methods* Pesticide Residues / analysis* Reproducibility of Results Tandem Mass Spectrometry / methods* |
| Chemical | |
Reg. No./Substance:
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0/Pesticide Residues |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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