| Perturbed bioelectrical properties of the mouse cecum following hepatectomy and starvation: the role of bacterial adherence. | |
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MedLine Citation:
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PMID: 10485604 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Previous work in our laboratory has demonstrated that bacterial adherence alone to the intestinal epithelium, as occurs following catabolic stress, significantly perturbs the normal electrophysiology of the cecal mucosa. The aim of this study was to further characterize these effects in the mouse cecum following hepatectomy and short-term starvation, and to define the role of bacterial adherence in this process. Groups of mice underwent a surgical hepatectomy and were either fed or starved during the postoperative period. Groups of controls underwent sham operations and were either fed or starved postoperatively. Electrophysiologic studies in Ussing chambers at 48 hours were performed. Bacterial adherence to the mucosa was assessed by culture and histologic staining. To determine the role of bacteria in the altered electrophysiologic response, ciprofloxacin decontamination studies were performed. Only mice subjected to both hepatectomy and starvation developed bacterial adherence of sufficient magnitude (>10(5) cfu/gm) to alter mucosal electrophysiology (short-circuit current and basal potential difference). Ciprofloxacin decontamination completely abrogated this effect. Ion replacement studies suggested that active sodium transport was primarily responsible for the observed changes in mucosal electrophysiology. Bacterial-epithelial cell interactions may be responsible for altered mucosal ion transport observed following operative catabolic stress and short-term starvation. |
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Authors:
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J C Alverdy; B Hendrickson; S S Guandalini; R J Laughlin; K Kent; R Banerjee |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Shock (Augusta, Ga.) Volume: 12 ISSN: 1073-2322 ISO Abbreviation: Shock Publication Date: 1999 Sep |
Date Detail:
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Created Date: 1999-11-03 Completed Date: 1999-11-03 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 9421564 Medline TA: Shock Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 235-41; discussion 242 Citation Subset: IM |
Affiliation:
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Department of Surgery and Pediatrics, University of Chicago, IL 60637, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bacterial Adhesion / physiology* Cecum / immunology, physiology* Electrophysiology Female Hepatectomy Immunoglobulin A / metabolism Intestinal Mucosa / immunology, physiology* Ion Transport Mice Mice, Inbred BALB C Starvation* |
| Grant Support | |
ID/Acronym/Agency:
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DK26678/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Immunoglobulin A |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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