Document Detail


Perturbations of amino acid metabolism associated with glyphosate-dependent inhibition of shikimic acid metabolism affect cellular redox homeostasis and alter the abundance of proteins involved in photosynthesis and photorespiration.
MedLine Citation:
PMID:  21757634     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The herbicide glyphosate inhibits the shikimate pathway of the synthesis of amino acids such as phenylalanine, tyrosine, and tryptophan. However, much uncertainty remains concerning precisely how glyphosate kills plants or affects cellular redox homeostasis and related processes in glyphosate-sensitive and glyphosate-resistant crop plants. To address this issue, we performed an integrated study of photosynthesis, leaf proteomes, amino acid profiles, and redox profiles in the glyphosate-sensitive soybean (Glycine max) genotype PAN809 and glyphosate-resistant Roundup Ready Soybean (RRS). RRS leaves accumulated much more glyphosate than the sensitive line but showed relatively few changes in amino acid metabolism. Photosynthesis was unaffected by glyphosate in RRS leaves, but decreased abundance of photosynthesis/photorespiratory pathway proteins was observed together with oxidation of major redox pools. While treatment of a sensitive genotype with glyphosate rapidly inhibited photosynthesis and triggered the appearance of a nitrogen-rich amino acid profile, there was no evidence of oxidation of the redox pools. There was, however, an increase in starvation-associated and defense proteins. We conclude that glyphosate-dependent inhibition of soybean leaf metabolism leads to the induction of defense proteins without sustained oxidation. Conversely, the accumulation of high levels of glyphosate in RRS enhances cellular oxidation, possibly through mechanisms involving stimulation of the photorespiratory pathway.
Authors:
Pedro Diaz Vivancos; Simon P Driscoll; Christopher A Bulman; Liu Ying; Kaveh Emami; Achim Treumann; Caroline Mauve; Graham Noctor; Christine H Foyer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-07-14
Journal Detail:
Title:  Plant physiology     Volume:  157     ISSN:  1532-2548     ISO Abbreviation:  Plant Physiol.     Publication Date:  2011 Sep 
Date Detail:
Created Date:  2011-09-05     Completed Date:  2012-01-30     Revised Date:  2013-06-28    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  256-68     Citation Subset:  IM    
Affiliation:
Centre for Plant Sciences, Faculty of Biology, University of Leeds, Leeds LS2 9JT, United Kingdom.
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / metabolism*
Glycine / analogs & derivatives*,  pharmacology
Herbicides / pharmacology*
Homeostasis*
Oxidation-Reduction
Photosynthesis*
Plant Proteins / metabolism*
Proteomics
Shikimic Acid / antagonists & inhibitors*,  metabolism
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Herbicides; 0/Plant Proteins; 1071-83-6/glyphosate; 138-59-0/Shikimic Acid; 56-40-6/Glycine
Comments/Corrections

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