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Perturbation of cell adhesion and microvilli formation by antisense oligonucleotides to ERM family members.
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MedLine Citation:
PMID:  8207064     Owner:  NLM     Status:  MEDLINE    
To examine the functions of ERM family members (ezrin, radixin, and moesin), mouse epithelial cells (MTD-1A cells) and thymoma cells (L5178Y), which coexpress all of them, were cultured in the presence of antisense phosphorothioate oligonucleotides (PONs) complementary to ERM sequences. Immunoblotting revealed that the antisense PONs selectively suppressed the expression of each member. Immunofluorescence microscopy of these ezrin, radixin, or moesin "single-suppressed" MTD-1A cells revealed that the ERM family members are colocalized at cell-cell adhesion sites, microvilli, and cleavage furrows, where actin filaments are densely associated with plasma membranes. The ezrin/radixin/moesin antisense PONs mixture induced the destruction of both cell-cell and cell-substrate adhesion, as well as the disappearance of microvilli. Ezrin or radixin antisense PONs individually affected the initial step of the formation of both cell-cell and cell-substrate adhesion, but did not affect the microvilli structures. In sharp contrast, moesin antisense PONs did not singly affect cell-cell and cell-substrate adhesion, whereas it partly affected the microvilli structures. These data indicate that ezrin and radixin can be functionally substituted, that moesin has some synergetic functional interaction with ezrin and radixin, and that these ERM family members are involved in cell-cell and cell-substrate adhesion, as well as microvilli formation.
K Takeuchi; N Sato; H Kasahara; N Funayama; A Nagafuchi; S Yonemura; S Tsukita; S Tsukita
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of cell biology     Volume:  125     ISSN:  0021-9525     ISO Abbreviation:  J. Cell Biol.     Publication Date:  1994 Jun 
Date Detail:
Created Date:  1994-07-12     Completed Date:  1994-07-12     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0375356     Medline TA:  J Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1371-84     Citation Subset:  IM    
Department of Information Physiology, National Institute for Physiological Sciences, Aichi, Japan.
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MeSH Terms
Base Sequence
Blood Proteins / genetics
Cell Adhesion / drug effects,  physiology*
Cytoskeletal Proteins*
DNA-Binding Proteins / genetics*
Fluorescent Antibody Technique
Leukemia L5178
Membrane Proteins / genetics
Microfilament Proteins*
Microscopy, Electron, Scanning
Microvilli / drug effects,  physiology*,  ultrastructure
Molecular Sequence Data
Oligonucleotides, Antisense / pharmacology*
Phosphoproteins / genetics
Proteins / genetics
Thionucleotides / pharmacology
Thymoma / ultrastructure
Transcription Factors / genetics*
Reg. No./Substance:
0/Blood Proteins; 0/Cytoskeletal Proteins; 0/DNA-Binding Proteins; 0/Etv5 protein, mouse; 0/Membrane Proteins; 0/Microfilament Proteins; 0/Oligonucleotides, Antisense; 0/Phosphoproteins; 0/Proteins; 0/Thionucleotides; 0/Transcription Factors; 0/ezrin; 144131-77-1/moesin; 144517-21-5/radixin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): J Cell Biol
Journal ID (publisher-id): J. Cell Biol.
ISSN: 0021-9525
ISSN: 1540-8140
Publisher: The Rockefeller University Press
Article Information
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Print publication date: Day: 2 Month: 6 Year: 1994
Volume: 125 Issue: 6
First Page: 1371 Last Page: 1384
ID: 2290919
Publisher Id: 94266967
PubMed Id: 8207064

Perturbation of cell adhesion and microvilli formation by antisense oligonucleotides to ERM family members

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