| Perturbation of NgTRF1 expression induces apoptosis-like cell death in tobacco BY-2 cells and implicates NgTRF1 in the control of telomere length and stability. | |
| | |
MedLine Citation:
|
PMID: 15528297 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Telomeres are specialized nucleoprotein complexes that are essential for preserving chromosome integrity in eukaryotic cells. Several potential telomere binding proteins have recently been identified in higher plants, but nothing is known about their in vivo functions. We previously identified NgTRF1 as a double-stranded telomeric repeat binding factor in tobacco (Nicotiana tabacum) and here show that the binding of NgTRF1 to telomeric repeats inhibits telomerase-mediated telomere extension. To determine whether NgTRF1 is involved in telomere length regulation, we established transgenic tobacco BY-2 cell lines that overexpress or suppress NgTRF1. Pulsed-field gel electrophoresis showed that 35S::NgTRF1 cells exhibited significantly shortened telomeres (45 to 10 kb), whereas 35S::antisense-NgTRF1 cells contained longer telomeres (80 to 25 kb) compared with wild-type and 35S::GUS control cells (65 to 15 kb), indicating that telomere length inversely correlates with the amount of functional NgTRF1 in BY-2 cells. 35S::NgTRF1 cells with shorter telomeres displayed a progressive reduction in cell viability and stopped dividing after 25 to 40 successive rounds of 12-d batch subculture, in sharp contrast with control cells, which have an unlimited capacity for division. Internucleosomal DNA fragmentation, mitochondrial release of cytochrome c, and terminal deoxynucleotidyltransferase-mediated dUTP nick end labeling positive nuclei were detected in 35S::NgTRF1 cells during prolonged subculture, indicating that enhanced cell death was attributable to an apoptosis-like mechanism. 35S::antisense-NgTRF1 cells containing low levels of NgTRF1 also exhibited a progressive decrease in cell viability and apoptotic cell death, but less so than did 35S::NgTRF1 cells, suggesting that the level of NgTRF1 is critically associated with cell viability. Taken together, these data indicate that perturbation of NgTRF1 expression results in changes in telomere length and stability, which in turn causes apoptotic cell death in transgenic BY-2 cells. These results are discussed in light of the suggestion that NgTRF1 is involved in the mechanism by which telomere length and stability are maintained. We further suggest that the structural stability of telomeres, in addition to length maintenance, is essential for their function and for the immortality of BY-2 cells. |
| | |
Authors:
|
Seong Wook Yang; Sung Keun Kim; Woo Taek Kim |
Related Documents
:
|
9849487 - Inhibition of human telomerase by a retrovirus expressing telomeric antisense rna. 8246307 - The effect of food chemicals on cell aging of human diploid cells in in vitro culture. 19578747 - Htert promotes the invasion of telomerase-negative tumor cells in vitro. 12143927 - Age does not influence dna fragmentation in the hippocampus after fatal traumatic brain... 10097167 - Gene disruption of p27(kip1) allows cell proliferation in the postnatal and adult organ... 229267 - Membrane proteins specified by herpes simplex viruses. v. identification of an fc-bindi... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2004-11-04 |
Journal Detail:
|
Title: The Plant cell Volume: 16 ISSN: 1040-4651 ISO Abbreviation: Plant Cell Publication Date: 2004 Dec |
Date Detail:
|
Created Date: 2004-12-06 Completed Date: 2005-06-24 Revised Date: 2009-11-18 |
Medline Journal Info:
|
Nlm Unique ID: 9208688 Medline TA: Plant Cell Country: United States |
Other Details:
|
Languages: eng Pagination: 3370-85 Citation Subset: IM |
Affiliation:
|
Department of Biology, College of Science, Yonsei University, Seoul 120-749, Korea. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Apoptosis
/
genetics* Cell Line, Transformed / metabolism Cell Nucleus / genetics Cell Survival / genetics DNA Fragmentation / genetics Gene Expression Regulation, Plant / genetics* Plant Proteins / genetics, metabolism* Plants, Genetically Modified / genetics Telomerase / metabolism Telomere / genetics* Telomeric Repeat Binding Protein 1 / genetics, metabolism* Tobacco / genetics, metabolism* Transgenes / genetics |
| Chemical | |
Reg. No./Substance:
|
0/Plant Proteins; 0/Telomeric Repeat Binding Protein 1; EC 2.7.7.49/Telomerase |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Characterization of novel monoclonal antibodies for prostate-specific antigen (PSA) with potency to ...
Next Document: Structural insights into the target specificity of plant invertase and pectin methylesterase inhibit...