Document Detail

Persistent spreading of ligament cells on osteopontin/bone sialoprotein-I or collagen enhances tolerance to heat shock.
MedLine Citation:
PMID:  2328768     Owner:  NLM     Status:  MEDLINE    
Fibronectin (FN), bone sialoprotein-I (BSP-I), Type I collagen, and a number of synthetic peptides containing the integrin attachment sequence (RDG) were evaluated for their ability to affect stress tolerance in osteo-ligament cells (OL). The attachment and spreading of OL cells was determined by the method of Klebe (1974) and Akiyama et al. (1986). Survival from heat shock was evaluated after the methods of Gerner et al. (1976). These studies showed that FN, BSP-I, and synthetic RGD peptides enhance attachment of OL cells. Increased survival from heat was limited to cells spread on fibronectin, BSP-I, and Type I collagen. OL cells that persistently spread on BSP-I and Type I collagen had more survivors than cells demonstrating transient spreading on FN. These studies indicate that (a) cell spreading is a prerequisite for stress tolerance and (b) enhanced stress tolerance is mediated by protein sequences other than those immediately surrounding the RGD sites in native proteins.
J J Sauk; C L Van Kampen; K Norris; J Moehring; R A Foster; M J Somerman
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Experimental cell research     Volume:  188     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  1990 May 
Date Detail:
Created Date:  1990-05-25     Completed Date:  1990-05-25     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  105-10     Citation Subset:  IM    
Department of Pathology, Dental School, University of Maryland, Baltimore 21201.
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MeSH Terms
Cell Movement / physiology
Cells, Cultured
Collagen / diagnostic use,  physiology*
Extracellular Matrix / physiology
Heat-Shock Proteins / physiology*
Ligaments / cytology*,  physiology
Sialoglycoproteins / diagnostic use,  physiology*
Grant Support
Reg. No./Substance:
0/Heat-Shock Proteins; 0/Sialoglycoproteins; 106441-73-0/Osteopontin; 9007-34-5/Collagen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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