Document Detail


Persistent mucus accumulation: a consequence of delayed bronchial mucous cell apoptosis in RAO-affected horses?
MedLine Citation:
PMID:  16500947     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study examined the contribution of delayed apoptosis of bronchial mucous cells to mucus accumulation in equine recurrent airway obstruction (RAO). In pilot studies, Bcl-2, an apoptosis inhibitor, was detected in airway mucous cells of RAO-affected horses in remission and during acute disease, when most mucus was secreted. To study whether delayed apoptosis results in an increase in the number of mucous cells during disease recovery, six RAO-affected and six control horses were fed hay for 5 days to induce inflammation and then pellets for 7 days to partially resolve RAO before euthanasia. RAO-affected horses had more airway obstruction and luminal mucus than control horses under both management systems. At the time of euthanasia, RAO-affected horses had more inflammation and Bcl-2-positive bronchial mucous cells than control animals. In horses with >10 and <10 neutrophils per microliter of bronchoalveolar lavage fluid, >50% and <10% of mucous cells stained positive for Bcl-2, respectively. No differences in mucous cell number or amount of stored mucosubstance were observed between RAO-affected and control horses, but in RAO-affected animals, the amount of stored mucosubstance decreased as the number of neutrophils in bronchoalveolar lavage fluid increased. Because the number of mucous cells was similar in both groups of horses but only mucous cells of RAO-affected horses expressed Bcl-2 during recovery from acute disease, a conclusive role for Bcl-2 in prolonging bronchial mucous cell life could not be determined. Future studies are needed to compare horses that are kept in remission for prolonged periods when all mucous cells are fully developed.
Authors:
Lisa R Bartner; N Edward Robinson; Matti Kiupel; Yohannes Tesfaigzi
Related Documents :
22618587 - Intrastriatal b-cell administration limits infarct size after stroke in b-cell deficien...
11078807 - Sodium butyrate induces apoptosis in human renal cell carcinoma cells and synergistical...
21036697 - Inhibition of bcl-2 in small cell lung cancer cell lines with oblimersen, an antisense ...
20051817 - The effects of stichopus japonicus acid mucopolysaccharide on the apoptosis of the huma...
3030867 - Effect of various polybrominated biphenyls on cell-cell communication in cultured human...
8020607 - Intercellular communication is reduced by tpa and ki-ras p21 in quiescent, but not prol...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2006-02-24
Journal Detail:
Title:  American journal of physiology. Lung cellular and molecular physiology     Volume:  291     ISSN:  1040-0605     ISO Abbreviation:  Am. J. Physiol. Lung Cell Mol. Physiol.     Publication Date:  2006 Oct 
Date Detail:
Created Date:  2006-09-08     Completed Date:  2006-11-07     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  100901229     Medline TA:  Am J Physiol Lung Cell Mol Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  L602-9     Citation Subset:  IM    
Affiliation:
Department of Large Animal Clinical Sciences, Michigan State University, East Lansing, MI 48824, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Airway Obstruction / veterinary*
Animals
Apoptosis*
Bronchi / metabolism,  pathology,  physiopathology*
Bronchoalveolar Lavage Fluid / cytology
Horse Diseases / metabolism,  physiopathology*
Horses
Metaplasia
Mucus / metabolism*
Proto-Oncogene Proteins c-bcl-2 / metabolism
Recurrence
Respiratory Mucosa / metabolism,  pathology,  physiopathology*
Time Factors
Grant Support
ID/Acronym/Agency:
ES-09237/ES/NIEHS NIH HHS; HL-68111/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Proto-Oncogene Proteins c-bcl-2

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Neither SP-A nor NH2-terminal domains of SP-A can substitute for SP-D in regulation of alveolar home...
Next Document:  Single nucleotide polymorphism in 5'-flanking region reduces transcription of surfactant protein B g...