| Persistent induction of somatic reversions of the pink-eyed unstable mutation in F1 mice born to fathers irradiated at the spermatozoa stage. | |
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MedLine Citation:
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PMID: 12005545 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Untargeted mutation and delayed mutation are features of radiation-induced genomic instability and have been studied extensively in tissue culture cells. The mouse pink-eyed unstable (p(un)) mutation is due to an intragenic duplication of the pink-eyed dilution locus and frequently reverts back to the wild type in germ cells as well as in somatic cells. The reversion event can be detected in the retinal pigment epithelium as a cluster of pigmented cells (eye spot). We have investigated the reversion p(um) in F1 mice born to irradiated males. Spermatogonia-stage irradiation did not affect the frequency of the reversion in F1 mice. However, 6 Gy irradiation at the spermatozoa stage resulted in an approximately twofold increase in the number of eye spots in the retinal pigment epithelium of F1 mice. Somatic reversion occurred for the paternally derived p(un) alleles. In addition, the reversion also occurred for the maternally derived, unirradiated p(un) alleles at a frequency equal to that for the paternally derived allele. Detailed analyses of the number of pigmented cells per eye spot indicated that the frequency of reversion was persistently elevated during the proliferation cycle of the cells in the retinal pigment epithelium when the male parents were irradiated at the spermatozoa stage. The present study demonstrates the presence of a long-lasting memory of DNA damage and the persistent up-regulation of recombinogenic activity in the retinal pigment epithelium of the developing fetus. |
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Authors:
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Kazunori Shiraishi; Tsutomu Shimura; Masataka Taga; Norio Uematsu; Yoichi Gondo; Megu Ohtaki; Ryo Kominami; Ohtsura Niwa |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Radiation research Volume: 157 ISSN: 0033-7587 ISO Abbreviation: Radiat. Res. Publication Date: 2002 Jun |
Date Detail:
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Created Date: 2002-05-13 Completed Date: 2002-06-19 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0401245 Medline TA: Radiat Res Country: United States |
Other Details:
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Languages: eng Pagination: 661-7 Citation Subset: IM; S |
Affiliation:
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Department of Late Effect Studies, Radiation Biology Center, Kyoto University, Yoshida Konoe, Sakyo-ku, Kyoto 606-8501, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Carrier Proteins* Crosses, Genetic DNA Damage / genetics, radiation effects* Embryonic and Fetal Development / radiation effects Eye / metabolism, pathology*, radiation effects* Female Gene Frequency Genotype Male Membrane Proteins / genetics* Mice Mice, Inbred C3H Mice, Inbred C57BL Mutation / genetics*, radiation effects Phenotype Pigment Epithelium of Eye / metabolism, pathology, radiation effects Spermatozoa / metabolism, radiation effects* Suppression, Genetic / genetics, radiation effects |
| Chemical | |
Reg. No./Substance:
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0/Carrier Proteins; 0/Membrane Proteins; 148710-77-4/P protein, mouse |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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