Document Detail

Persistent induction of somatic reversions of the pink-eyed unstable mutation in F1 mice born to fathers irradiated at the spermatozoa stage.
MedLine Citation:
PMID:  12005545     Owner:  NLM     Status:  MEDLINE    
Untargeted mutation and delayed mutation are features of radiation-induced genomic instability and have been studied extensively in tissue culture cells. The mouse pink-eyed unstable (p(un)) mutation is due to an intragenic duplication of the pink-eyed dilution locus and frequently reverts back to the wild type in germ cells as well as in somatic cells. The reversion event can be detected in the retinal pigment epithelium as a cluster of pigmented cells (eye spot). We have investigated the reversion p(um) in F1 mice born to irradiated males. Spermatogonia-stage irradiation did not affect the frequency of the reversion in F1 mice. However, 6 Gy irradiation at the spermatozoa stage resulted in an approximately twofold increase in the number of eye spots in the retinal pigment epithelium of F1 mice. Somatic reversion occurred for the paternally derived p(un) alleles. In addition, the reversion also occurred for the maternally derived, unirradiated p(un) alleles at a frequency equal to that for the paternally derived allele. Detailed analyses of the number of pigmented cells per eye spot indicated that the frequency of reversion was persistently elevated during the proliferation cycle of the cells in the retinal pigment epithelium when the male parents were irradiated at the spermatozoa stage. The present study demonstrates the presence of a long-lasting memory of DNA damage and the persistent up-regulation of recombinogenic activity in the retinal pigment epithelium of the developing fetus.
Kazunori Shiraishi; Tsutomu Shimura; Masataka Taga; Norio Uematsu; Yoichi Gondo; Megu Ohtaki; Ryo Kominami; Ohtsura Niwa
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Radiation research     Volume:  157     ISSN:  0033-7587     ISO Abbreviation:  Radiat. Res.     Publication Date:  2002 Jun 
Date Detail:
Created Date:  2002-05-13     Completed Date:  2002-06-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0401245     Medline TA:  Radiat Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  661-7     Citation Subset:  IM; S    
Department of Late Effect Studies, Radiation Biology Center, Kyoto University, Yoshida Konoe, Sakyo-ku, Kyoto 606-8501, Japan.
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MeSH Terms
Carrier Proteins*
Crosses, Genetic
DNA Damage / genetics,  radiation effects*
Embryonic and Fetal Development / radiation effects
Eye / metabolism,  pathology*,  radiation effects*
Gene Frequency
Membrane Proteins / genetics*
Mice, Inbred C3H
Mice, Inbred C57BL
Mutation / genetics*,  radiation effects
Pigment Epithelium of Eye / metabolism,  pathology,  radiation effects
Spermatozoa / metabolism,  radiation effects*
Suppression, Genetic / genetics,  radiation effects
Reg. No./Substance:
0/Carrier Proteins; 0/Membrane Proteins; 148710-77-4/P protein, mouse

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