| Persistence of virulent Leishmania major in murine cutaneous leishmaniasis: a possible hazard for the host. | |
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MedLine Citation:
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PMID: 8093358 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The persistence of Leishmania major parasites in mice resistant to infection was investigated by the polymerase chain reaction and in vitro culture methods. Parasite-specific DNA was detected in the lymph nodes, spleens, bone marrow, and livers of C57BL/6 mice 1 year after their recovery from infection. Live parasites were also recovered from these tissues (except liver tissues) and were used to establish in vitro isolates. Pulsed-field gel electrophoresis, Southern blotting, and Western blot (immunoblot) analyses showed that these isolates retained the karyotype and the phenotype of the original inoculum, including the levels of expression of gp63 and lipophosphoglycan, the two major surface molecules of Leishmania species. More importantly, these isolates were virulent and induced fatal disease when injected into susceptible BALB/c mice. Persistence was shown to be a more general phenomenon, since several different strains of mice which were resistant to L. major infection also harbored persistent parasites. The implications for the etiology of human leishmaniasis in immunocompromised individuals such as AIDS patients are discussed. |
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Authors:
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T Aebischer; S F Moody; E Handman |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Infection and immunity Volume: 61 ISSN: 0019-9567 ISO Abbreviation: Infect. Immun. Publication Date: 1993 Jan |
Date Detail:
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Created Date: 1993-01-29 Completed Date: 1993-01-29 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0246127 Medline TA: Infect Immun Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 220-6 Citation Subset: IM |
Affiliation:
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Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Victoria, Australia. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Base Sequence Blotting, Southern Blotting, Western Bone Marrow / parasitology Disease Models, Animal Immune Tolerance* Kidney / parasitology Leishmania tropica / pathogenicity* Leishmaniasis, Cutaneous / parasitology* Liver / parasitology Lymph Nodes / parasitology Mice Mice, Inbred BALB C Mice, Inbred C57BL Molecular Sequence Data Polymorphism, Restriction Fragment Length Spleen / parasitology Time Factors Virulence |
| Grant Support | |
ID/Acronym/Agency:
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R01-AI19347/AI/NIAID NIH HHS |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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