Document Detail


Persistence of virulent Leishmania major in murine cutaneous leishmaniasis: a possible hazard for the host.
MedLine Citation:
PMID:  8093358     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The persistence of Leishmania major parasites in mice resistant to infection was investigated by the polymerase chain reaction and in vitro culture methods. Parasite-specific DNA was detected in the lymph nodes, spleens, bone marrow, and livers of C57BL/6 mice 1 year after their recovery from infection. Live parasites were also recovered from these tissues (except liver tissues) and were used to establish in vitro isolates. Pulsed-field gel electrophoresis, Southern blotting, and Western blot (immunoblot) analyses showed that these isolates retained the karyotype and the phenotype of the original inoculum, including the levels of expression of gp63 and lipophosphoglycan, the two major surface molecules of Leishmania species. More importantly, these isolates were virulent and induced fatal disease when injected into susceptible BALB/c mice. Persistence was shown to be a more general phenomenon, since several different strains of mice which were resistant to L. major infection also harbored persistent parasites. The implications for the etiology of human leishmaniasis in immunocompromised individuals such as AIDS patients are discussed.
Authors:
T Aebischer; S F Moody; E Handman
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Infection and immunity     Volume:  61     ISSN:  0019-9567     ISO Abbreviation:  Infect. Immun.     Publication Date:  1993 Jan 
Date Detail:
Created Date:  1993-01-29     Completed Date:  1993-01-29     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  220-6     Citation Subset:  IM    
Affiliation:
Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Victoria, Australia.
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MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
Blotting, Southern
Blotting, Western
Bone Marrow / parasitology
Disease Models, Animal
Immune Tolerance*
Kidney / parasitology
Leishmania tropica / pathogenicity*
Leishmaniasis, Cutaneous / parasitology*
Liver / parasitology
Lymph Nodes / parasitology
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Molecular Sequence Data
Polymorphism, Restriction Fragment Length
Spleen / parasitology
Time Factors
Virulence
Grant Support
ID/Acronym/Agency:
R01-AI19347/AI/NIAID NIH HHS
Comments/Corrections

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