Document Detail


Persistence of sister chromatid exchange by 9-beta-D-arabinofuranosyladenine in Chinese hamster ovary cells cultivated in vitro.
MedLine Citation:
PMID:  8231826     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The cytogenetic activity of 9-beta-D-arabinofuranosyladenine (ara-A), a known antitumor agent with an unknown mechanism of action (possibly acting via demethylation and/or decrease in DNA synthesis), has been tested in Chinese hamster ovary (CHO) cells cultivated in vitro, with respect to its ability to induce sister chromatid exchange (SCE). The agent shows no effect on cell cycle parameters (proliferation and mitotic indices) at concentrations up to 100 microM, when administered in pulses ranging from 3 to 12 h. Furthermore, even if administered over the entire treatment period (24 h), a severe cell cycle delay appears only at a concentration of 100 microM. A clear increase in the SCE frequency is produced starting from a concentration of 10 microM, irrespective of the treatment protocol (i.e. pulse versus continuous). This effect on the SCE frequency is maintained for as long as 10 cell cycles after removal of the agent. A partial inactivation of S-adenosyl-L-homocysteine (AdoHcy) hydrolase seems to be produced for as long as 6 h after agent removal. We suggest that under our experimental conditions ara-A exerts its action through a heritable epigenetic modification.
Authors:
P Perticone; M Linguardo; R Cozzi; R M Corbo; S Polani
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Mutagenesis     Volume:  8     ISSN:  0267-8357     ISO Abbreviation:  Mutagenesis     Publication Date:  1993 Sep 
Date Detail:
Created Date:  1993-12-01     Completed Date:  1993-12-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8707812     Medline TA:  Mutagenesis     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  445-8     Citation Subset:  IM    
Affiliation:
Centro di Genetica Evoluzionistica del CNR, Roma, Italy.
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MeSH Terms
Descriptor/Qualifier:
Adenosylhomocysteinase
Animals
CHO Cells
Cell Cycle
Cell Division / drug effects
Cricetinae
Cricetulus
Dose-Response Relationship, Drug
Germ-Line Mutation
Hydrolases / antagonists & inhibitors
Mitotic Index
Sister Chromatid Exchange*
Time Factors
Vidarabine / administration & dosage,  toxicity*
Chemical
Reg. No./Substance:
5536-17-4/Vidarabine; EC 3.-/Hydrolases; EC 3.3.1.1/Adenosylhomocysteinase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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