Document Detail

Persistence of nucleolar RNA-rich structures and Ph1 duplication in the blastic crisis of chronic myeloid leukaemia.
MedLine Citation:
PMID:  94687     Owner:  NLM     Status:  MEDLINE    
Nucleolar persistence in metaphase plates is a feature observed in most of the cells in neoplastic processes. Pathological persistence or fragmentation of the nucleoli is thought to be the cause of some numerical chromosomal aberrations due to non-disjunction of the chromatids, with particular involvement of the satellite chromosomes. Thus, a combined selective staining of both the nucleoli (amido black 10B according to Mundkur and Brauer's cytochemical technique) and the chromosomes (neutral red) was applied to the metaphase plates of patients with chronic myeloid leukaemia in the blastic crisis. Duplicated Ph1 was associated with amido black-stained areas at a very high rate in some cases. Since the blastic crisis in chronic myeloid leukaemia is characterized by the appearance of an increased number of immature, highly nucleolated cells, these findings lend support to the hypothesis that the duplication of the Ph1 represents a feature possibly favoured by the pathological persistence of nucleolar RNA-rich structures in the metaphase.
G Castoldi; G Borsetti; V Abbasciano; G Scapoli
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  La Ricerca in clinica e in laboratorio     Volume:  9     ISSN:  0390-5748     ISO Abbreviation:  Ric Clin Lab     Publication Date:    1979 Jul-Sep
Date Detail:
Created Date:  1980-07-12     Completed Date:  1980-07-12     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  7613947     Medline TA:  Ric Clin Lab     Country:  ITALY    
Other Details:
Languages:  eng     Pagination:  265-71     Citation Subset:  IM    
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MeSH Terms
Bone Marrow Cells
Cell Nucleolus / analysis*
Chromosome Aberrations*
Chromosome Disorders*
Chromosomes, Human, 21-22 and Y*
Leukemia, Myeloid*
Leukemia, Myeloid, Acute*
Middle Aged
RNA / analysis*
Reg. No./Substance:

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