Document Detail


Persistence of memory type lymphocytes in an experimental model of chronic pancreatitis in rats.
MedLine Citation:
PMID:  12030436     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
There is growing evidence that immunocompetent cells are involved in the pathogenesis of chronic pancreatitis. Using a model of chronic pancreatitis induced by dibutyltin dichloride (DBTC) in rats, we have immunohistochemically phenotyped the infiltrating T lymphocytes, CD45RC+ cells, macrophages, as well as the IL-2 receptor as an activation marker during a time course of two months. In addition, the expression of CD44, of the integrin component CD18, and of MHC class II was determined. Furthermore, the isoforms of CD45 which are generated by alternative mRNA splicing were analysed using reverse transcription followed by the polymerase chain reaction-technique (RT-PCR). The pattern of the local leukocytes in the DBTC pancreatitis suggests that the acute unspecific inflammation is followed by an activation of T lymphocytes resulting in the chronic course of the disease.
Authors:
Gisela Sparmann; Sabine Sass; Hans-Joachim Böhme; Stefan Liebe; Jörg Emmrich
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cellular and molecular biology (Noisy-le-Grand, France)     Volume:  48     ISSN:  0145-5680     ISO Abbreviation:  Cell. Mol. Biol. (Noisy-le-grand)     Publication Date:  2002 May 
Date Detail:
Created Date:  2002-05-27     Completed Date:  2003-08-05     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9216789     Medline TA:  Cell Mol Biol (Noisy-le-grand)     Country:  France    
Other Details:
Languages:  eng     Pagination:  309-15     Citation Subset:  IM    
Affiliation:
Department of Internal Medicine, University Rostock, Germany. gisela.sparmann@med.uni-rostock.de
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MeSH Terms
Descriptor/Qualifier:
Alternative Splicing
Animals
Antigens, CD18 / metabolism
Antigens, CD45
Chronic Disease
Disease Models, Animal
Ethidium
Genes, MHC Class II
Immunohistochemistry
Immunologic Memory*
Macrophages / metabolism
Male
Organotin Compounds
Pancreatitis / immunology*
Protein Isoforms
Rats
Rats, Inbred Lew
Receptors, Interleukin-2 / metabolism
T-Lymphocytes / immunology*
Chemical
Reg. No./Substance:
0/Antigens, CD18; 0/Organotin Compounds; 0/Protein Isoforms; 0/Receptors, Interleukin-2; 3546-21-2/Ethidium; 683-18-1/dibutyldichlorotin; EC 3.1.3.48/Antigens, CD45

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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