| Persistence of herpes simplex virus genes in cells of neuronal origin. | |
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MedLine Citation:
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PMID: 6251268 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The growth characteristics of the KOS strain of herpes simplex virus type 1 (HSV-1) in cell lines of nervous tissues origin were examined in an attempt to develop a tissue culture system mimicking the in vivo state of HSV-1 latency. We have previously reported that the B103 rat brain neuroma cell line is nonpermissive for growth of the KOS strain. In this report, we show that this nonpermissiveness is a temperature- and multiplicity-dependent phenomenon, with minimum virus yields at an elevated temperature and a low multiplicity of infection. Under these conditions, B103 cells survived infection with active wild-type or mutant HSV-1, whereas similarly treated Vero cells were killed. Six independent cultures of B103 cells surviving HSV-1 infection have been established. The surviving cells ceased production of any HSV-1 virus by 14 days postinfection and resumed growth and division at rates comparable to those of uninfected B103 cells. Survivor cells continued to express HSV-1-specific antigens, however, as detected by indirect immunofluorescence and by surface iodination followed by immunoprecipitation and polyacrylamide gel electrophoresis. The survivor cells did not express all of the surface proteins seen on productively infected B103 cells, and they were not susceptible to complement-mediated immune cytolysis with anti-HSV-1 antiserum. These results demonstrate that at least a portion of the HSV-1 genome is being harbored in these survivor cells. |
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Authors:
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M Levine; A L Goldin; J C Glorioso |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of virology Volume: 35 ISSN: 0022-538X ISO Abbreviation: J. Virol. Publication Date: 1980 Jul |
Date Detail:
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Created Date: 1980-11-20 Completed Date: 1980-11-20 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0113724 Medline TA: J Virol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 203-10 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antigens, Surface / analysis Antigens, Viral / analysis Carrier State Cell Survival Cells, Cultured Fluorescent Antibody Technique Haplorhini Mutation Neurons / microbiology* Rats Simplexvirus / genetics*, growth & development Temperature |
| Grant Support | |
ID/Acronym/Agency:
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05860//PHS HHS; 07711//PHS HHS; 5P40-RR00200/RR/NCRR NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Surface; 0/Antigens, Viral |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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