Document Detail


Persistence of gamma-glutamyl transpeptidase-positive foci during hamster buccal pouch carcinogenesis.
MedLine Citation:
PMID:  6143614     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This study describes the kinetics of induction and growth as well as the property of persistence of gamma-glutamyl transpeptidase (GGT)-stained cell populations (GGT-positive foci) induced in hamster buccal pouch epithelium by a 5-week regimen of biweekly topical applications of 7, 12-dimethylbenz(a)anthracene (DMBA). During such treatment, and at various times thereafter, GGT-positive foci were detected and quantitated in whole mounts of pouch epithelium stained histochemically for GGT activity. A comparison of GGT-positive foci at the completion of the DMBA regimen and at 10 weeks thereafter revealed no significant decrease in either the number or the size of the foci. During the same 10-week posttreatment period, several dysplastic and occasional neoplastic lesions, some of which displayed patchy GGT activity, developed in the pouch epithelium of DMBA-treated animals. Twenty-two hamsters were sacrificed between the 15th and 34th weeks of the experiment for histological and histochemical study. Fifteen of these animals had grossly visible epithelial neoplasms, and the 11 which could be evaluated using the whole-mount technique had a few residual GGT-positive foci. None of the 33 control animals evaluated at various times during the experiment developed any gross, microscopic, or GGT-positive lesions. These data indicate that, following a 5-week regimen of DMBA applications, an appreciable number of GGT-positive foci persist throughout the latent period, during which dysplastic and neoplastic buccal pouch lesions develop. The expression of GGT activity in early hyperplastic and dysplastic lesions and its persistence in several animals provide additional support for the hypothesis that GGT-positive foci may represent sites of subsequent neoplastic development.
Authors:
T Odajima; D B Solt; L C Solt
Related Documents :
14703054 - Population dynamics of culex quinquefasciatus filaria vector in raipur city of chhattis...
12805834 - Prevention and control of losses of gaseous nitrogen compounds in livestock operations:...
4415824 - Some factors involved in the comparison of response systems: acquisition, extinction, a...
21144024 - The spiritual distress assessment tool: an instrument to assess spiritual distress in h...
10412084 - The consumer assessment of health plan study (cahps) survey of children's health care.
21627724 - Successful chronic disease care for aboriginal australians requires cultural competence.
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cancer research     Volume:  44     ISSN:  0008-5472     ISO Abbreviation:  Cancer Res.     Publication Date:  1984 May 
Date Detail:
Created Date:  1984-06-01     Completed Date:  1984-06-01     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2984705R     Medline TA:  Cancer Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2062-7     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
9,10-Dimethyl-1,2-benzanthracene
Animals
Cheek
Cricetinae
Histocytochemistry
Kinetics
Male
Mesocricetus
Mouth Neoplasms / chemically induced,  enzymology*,  pathology
Precancerous Conditions / enzymology*,  pathology
gamma-Glutamyltransferase / metabolism*
Grant Support
ID/Acronym/Agency:
CA 00863/CA/NCI NIH HHS; CA 34160/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
57-97-6/9,10-Dimethyl-1,2-benzanthracene; EC 2.3.2.2/gamma-Glutamyltransferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Neurotransmitters in the basal ganglia.
Next Document:  Synthesis and transport of the organic matrix of the spicules in the gorgonian Leptogorgia virgulata...