| Persistence of ethyl carbamate-induced DNA damage in vivo as indicated by sister chromatid exchange analysis. | |
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MedLine Citation:
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PMID: 6825116 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Various treatment protocols were designed to investigate sister chromatid exchanges (SCEs) induced over successive posttreatment cell cycles in bone marrow and alveolar macrophage cells following treatment of C57BL/6J X DBA/2J F1 mice by i.p. injection of ethyl carbamate (3.3 mmol/kg). The same initial extent of alkylation in bone marrow and alveolar macrophages was suggested by identical SCE frequencies produced in both cell types by a one-cycle exposure protocol. The relatively lower responses in bone marrow cells by all other protocols may be a result of its faster mean population-cycling time. Second- and third-division cell SCE data produced by the various protocols indicate persistence of SCE-inducing lesions with no evidence of repair. In spite of the demonstrated lack of repair, first-cycle ethyl carbamate treatment was less effective than was second-cycle treatment in inducing SCEs. These results could not be attributed to selection of less-damaged cells over 2 cycles or to enhanced bromodeoxyuridine sensitivity in the second-cycle treatment protocol. It is speculated that the apparent cancellation of SCEs occurring over two successive cycles in the two-cycle exposure protocol may indicate the transient presence of ethyl carbamate-induced DNA interstrand cross-links. A possible mechanism of action of ethyl carbamate involving the formation of a transient cross-link and a persistent DNA monoadduct is postulated. |
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Authors:
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M K Conner; M Cheng |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Cancer research Volume: 43 ISSN: 0008-5472 ISO Abbreviation: Cancer Res. Publication Date: 1983 Mar |
Date Detail:
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Created Date: 1983-04-07 Completed Date: 1983-04-07 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2984705R Medline TA: Cancer Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 965-71 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bone Marrow / drug effects Bone Marrow Cells Cell Cycle Cell Survival / drug effects Crossing Over, Genetic* DNA / analysis* Macrophages / cytology, drug effects Mathematics Mice Mice, Inbred C57BL Mice, Inbred DBA Sister Chromatid Exchange* Urethane / pharmacology* |
| Chemical | |
Reg. No./Substance:
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51-79-6/Urethane; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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