Document Detail

Persistence of Chlamydia pneumoniae in degenerative aortic valve stenosis indicated by heat shock protein 60 homologues.
MedLine Citation:
PMID:  12578339     Owner:  NLM     Status:  MEDLINE    
BACKGROUND AND AIMS OF THE STUDY: Based on the concept of chronic persistent infections with Chlamydia pneumoniae among variable stressors for aortic valve degeneration, the study aim was to assess the presence of chlamydial heat shock protein (cHSP) 60 and its human homologue (hHSP60) in diseased valvular tissue. METHODS: Surgical specimens of high-grade stenosed, native (n = 33) and bioprosthetic (n = 10) aortic valves were examined immunohistochemically for the localization of cHSP60, hHSP60 and macrophages (CD68), supplemented by polymerase chain reaction (PCR) and electron microscopy to prove microbial presence. RESULTS: Degenerated valves showed specific immunostaining of cHSP60 in 27 cases (65%), of hHSP60 in 26 (63%), and of CD68 in 36 (84%). Both HSP60 homologues were predominantly detected in valvular fibrosa, consistently co-localized with macrophages and, quantitatively, showed a strong correlation (r = 0.81, p < 0.001). Presence of C. pneumoniae was demonstrated by PCR in a subset of 11 of 18 valves (61%). Microbial persistence was confirmed by ultrastructural analysis. Degenerated prosthetic valves revealed markedly higher macrophage infiltration and cHSP60 signaling compared with degenerated native valves (each p < 0.05). CONCLUSION: Beyond detection of C. pneumoniae, the present data on co-localization and valvular predilection sites (fibrosa) of both HSP60 homologues indicate the presence of chronic persistent C. pneumoniae infection as well as regional stressor effects, and suggest their involvement in native and prosthetic valve degeneration.
Dirk Skowasch; Kristina Yeghiazaryan; Stephanie Schrempf; Olga Golubnitschaja; Ulrich Welsch; Claus J Preusse; James A Likungu; Armin Welz; Berndt Lüderitz; Gerhard Bauriedel
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of heart valve disease     Volume:  12     ISSN:  0966-8519     ISO Abbreviation:  J. Heart Valve Dis.     Publication Date:  2003 Jan 
Date Detail:
Created Date:  2003-02-11     Completed Date:  2003-04-30     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9312096     Medline TA:  J Heart Valve Dis     Country:  England    
Other Details:
Languages:  eng     Pagination:  68-75     Citation Subset:  IM    
Heart Center University of Bonn, Bonn, Germany.
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MeSH Terms
Aged, 80 and over
Aortic Valve / microbiology
Aortic Valve Stenosis / epidemiology,  microbiology*
Chaperonin 60 / isolation & purification,  metabolism*
Chlamydophila Infections / complications
Chlamydophila pneumoniae*
Coronary Artery Disease / epidemiology,  microbiology
Fungal Proteins / isolation & purification,  metabolism*
Middle Aged
Polymerase Chain Reaction
Reg. No./Substance:
0/Chaperonin 60; 0/Fungal Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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