Document Detail

Peroxynitrite induces apoptosis in rat aortic smooth muscle cells: possible relation to vascular diseases.
MedLine Citation:
PMID:  14988519     Owner:  NLM     Status:  MEDLINE    
An emerging body of evidence is accumulating to suggest that in vivo formation of free radicals in the vasculature, such as peroxynitrite (ONOO-), and programmed cell death (i.e., apoptosis) play important roles in vascular diseases such as atherosclerosis, hypertension, and restenosis. The present study was designed to determine whether primary rat aortic smooth muscle cells (SMCs) undergo apoptosis following treatment with ONOO-. Direct exposure of primary rat aortic SMCs to ONOO--induced apoptosis in a concentration-dependent manner, as confirmed by means of quantitative fluorescence staining and TUNEL assays. ONOO--induced apoptosis in rat aortic SMCs appears to involve activation of Ca2+-dependent endonucleases. Although the precise mechanisms by which peroxynitrite induces apoptosis in rat aortic SMCs need to be further investigated, the present, preliminary findings could be used to suggest that ONOO- formation in the vasculature may play roles in the processes of vascular diseases, such as atherosclerosis, hypertension, and restenosis, via adverse actions on blood vessels.
Jianfeng Li; Wenyan Li; Jialin Su; Weimin Liu; Bella T Altura; Burton M Altura
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Experimental biology and medicine (Maywood, N.J.)     Volume:  229     ISSN:  1535-3702     ISO Abbreviation:  Exp. Biol. Med. (Maywood)     Publication Date:  2004 Mar 
Date Detail:
Created Date:  2004-02-27     Completed Date:  2004-03-24     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  100973463     Medline TA:  Exp Biol Med (Maywood)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  264-9     Citation Subset:  IM    
Department of Physiology, State University of New York, Downstate Medical Center, Brooklyn, New York 11203, USA.
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MeSH Terms
Aorta / drug effects
Apoptosis / drug effects*
Calcium / metabolism
Cell Survival / drug effects
Cells, Cultured
Dose-Response Relationship, Drug
Endonucleases / drug effects,  metabolism
In Situ Nick-End Labeling
Microscopy, Fluorescence
Muscle, Smooth, Vascular / drug effects*,  pathology
Myocytes, Smooth Muscle / drug effects*,  pathology
Peroxynitrous Acid / pharmacology*
Rats, Wistar
Grant Support
Reg. No./Substance:
14691-52-2/Peroxynitrous Acid; 7440-70-2/Calcium; EC 3.1.-/Endonucleases

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