| Peroxynitrite diminishes gap junctional communication: protection by selenite supplementation. | |
| | |
MedLine Citation:
|
PMID: 10632565 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Loss of intercellular communication via gap junctions has been correlated with progression of cells to a malignant phenotype. Here, we show that peroxynitrite, a mediator of toxicity in inflammatory processes, diminishes gap junctional intercellular communication (GJIC) in WB-F344 rat liver epithelial cells, assayed by the scrapeloading dye-transfer technique as well as by microinjection of a fluorescent dye into single cells. Exposure of cultured cells to a steady-state concentration of peroxynitrite of 1.6 microM for 4 min or to 3-morpholinosydnonimine (SIN-1) at 0.5 mM strongly diminished GJIC. These concentrations of peroxynitrite or SIN-1 were not cytotoxic. When cells were grown in a medium supplemented with sodium selenite (0.1-1 microM) for 72 h, substantial protection was afforded against the decrease in GJIC by peroxynitrite. Thus, peroxynitrite can disrupt GJIC, and selenium-containing proteins protect. |
| | |
Authors:
|
V S Sharov; K Briviba; H Sies |
Related Documents
:
|
1963075 - Tyrosine phosphorylation of a gap junction protein correlates with inhibition of cell-t... 17428265 - Functional expression of connexin30 and connexin31 in the polarized human airway epithe... 10079515 - Quiescent mammary epithelial cells have reduced connexin43 but maintain a high level of... 17295145 - Effect of berberine on barrier function in a human retinal pigment epithelial cell line. 6365705 - Adherence of group b streptococci isolated from man and cattle to human and bovine epit... 9382935 - Regulatory volume increase in rat pancreatic beta-cells. |
Publication Detail:
|
Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: IUBMB life Volume: 48 ISSN: 1521-6543 ISO Abbreviation: IUBMB Life Publication Date: 1999 Oct |
Date Detail:
|
Created Date: 2000-02-09 Completed Date: 2000-02-09 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 100888706 Medline TA: IUBMB Life Country: ENGLAND |
Other Details:
|
Languages: eng Pagination: 379-84 Citation Subset: IM |
Affiliation:
|
Institut für Physiologische Chemie I, Heinrich-Heine-Universität Düsseldorf, Germany. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cell Communication / drug effects* Cell Division / drug effects, physiology Cells, Cultured Culture Media Dose-Response Relationship, Drug Epithelial Cells / cytology, drug effects, metabolism Gap Junctions / drug effects* Glutathione Peroxidase / biosynthesis Homeostasis / drug effects Liver / cytology, drug effects, metabolism Nitrates / toxicity* Oxidants / toxicity* Protein Biosynthesis Proteins* Rats Rats, Inbred F344 Selenoproteins Sodium Selenite / metabolism, pharmacology* |
| Chemical | |
Reg. No./Substance:
|
0/Culture Media; 0/Nitrates; 0/Oxidants; 0/Proteins; 0/Selenoproteins; 10102-18-8/Sodium Selenite; 26404-66-0/peroxynitric acid; EC 1.11.1.9/Glutathione Peroxidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Hypoxia, ischemic stroke, and memory deficits: prospects for therapy.
Next Document: Changes of glycosylation of serum proteins in Sjögren's syndrome: correlation with interleukin-6 an...