| Peroxynitrite-induced nitration of cyclooxygenase-2 and inducible nitric oxide synthase promotes their binding in diabetic angiopathy. | |
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MedLine Citation:
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PMID: 20607198 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) play crucial roles in diabetic angiopathy. In vivo, however, the following facts remain unknown: whether COX-2 and iNOS bind, how peroxynitrite-induced nitration of COX-2 and iNOS affects their binding if they do bind and what effects of this mechanism contribute to diabetic angiopathy. This study focused on the issues above. Diabetes was induced in Wistar male rats by intraperitoneal injection of streptozotocin. As a specific scavenger of peroxynitrite, urate was used. After 13 wks of diabetes, the morphological and biochemical changes of the rats showed obvious diabetic angiopathy. There exists in vivo colocalization and binding of COX-2 and iNOS in diabetic angiopathy. The nitration level of total and co-immunoprecipitated COX-2 and iNOS increased significantly, and, simultaneously, their binding and activity increased in the diabetes group. In the diabetes + urate group, the nitration level of COX-2 and iNOS decreased and their binding reduced, consistent with their decreased activity and the attenuated pathological changes in the rat aorta and glomerulus. The results provide in vivo evidence that COX-2 and iNOS can bind in diabetic angiopathy and that peroxynitrite-induced nitration of COX-2 and iNOS promotes their binding, contributing to diabetic angiopathy. |
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Authors:
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Yanning Li; Jinsheng Qi; Kun Liu; Bin Li; Hui Wang; Jinhai Jia |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-06-30 |
Journal Detail:
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Title: Molecular medicine (Cambridge, Mass.) Volume: 16 ISSN: 1528-3658 ISO Abbreviation: Mol. Med. Publication Date: 2010 Sep-Oct |
Date Detail:
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Created Date: 2010-09-09 Completed Date: 2010-12-22 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9501023 Medline TA: Mol Med Country: United States |
Other Details:
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Languages: eng Pagination: 335-42 Citation Subset: IM |
Affiliation:
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Department of Molecular Biology, Hebei Key Lab of Laboratory Animal. Hebei Medical University, 361 East Zhongshan Road, Shijiazhuang, PR China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Aorta / drug effects, enzymology, pathology Blood Glucose / metabolism Body Weight / drug effects Cyclooxygenase 2 / metabolism* Diabetic Angiopathies / blood, enzymology*, pathology Endothelium, Vascular / drug effects, enzymology, pathology Immunoprecipitation Kidney Cortex / drug effects, enzymology, pathology, ultrastructure Kidney Glomerulus / drug effects, enzymology, pathology, ultrastructure Male Nitric Oxide Synthase Type II / metabolism* Nitrosation / drug effects Peroxynitrous Acid / pharmacology* Protein Binding / drug effects Protein Transport / drug effects Rats Rats, Wistar |
| Chemical | |
Reg. No./Substance:
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0/Blood Glucose; 14691-52-2/Peroxynitrous Acid; EC 1.14.13.39/Nitric Oxide Synthase Type II; EC 1.14.13.39/Nos2 protein, rat; EC 1.14.99.1/Cyclooxygenase 2; EC 1.14.99.1/Ptgs2 protein, rat |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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