| Peroxovanadium-mediated protection against murine leishmaniasis: role of the modulation of nitric oxide. | |
| | |
MedLine Citation:
|
PMID: 11009089 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The phosphotyrosine phosphatase inhibitor bpV(phen) has the ability to markedly decrease the progression of leishmaniasis in vivo. Here, we have identified the mechanisms that are responsible for this protective effect. We report that two potent peroxovanadium (pV) compounds, bpV(phen) and bpV(pic), control progression of leishmaniasis in a similar manner by modulating NO-dependent microbicidal action. We observed that their injection can rapidly and transiently induce the expression of inducible NO synthase (iNOS) in livers of mice and enhance circulating nitrate levels. Treatment of mice with bpV(phen) or bpV(pic) completely controlled progression of leishmaniasis in an NO-dependent manner, since inhibition of iNOS with aminoguanidine completely reversed this pV-mediated protection. This NO-dependent pV-mediated protection was further demonstrated by the incapacity of bpV(phen)-treated Nramp-/-, iNOS-/- mutant mice to control Leishmania major infection. Using an air pouch model, we showed that bpV(phen) can strongly modulate secretion of L. major-induced pro-inflammatory molecules and neutrophil recruitment. In addition, we observed that bpV(phen) per se can strongly induce the expression of Th1 type cytokines over Th2 in spleens of animals. Overall, this study has allowed us to establish the in vivo functional and immunological events involved in pV-mediated protective mechanism against leishmaniasis and that NO plays a pivotal role in this process. |
| | |
Authors:
|
C Matte; J F Marquis; J Blanchette; P Gros; R Faure; B I Posner; M Olivier |
Related Documents
:
|
17126589 - Inos expression is stimulated by the major surface protein (rwsp) from wolbachia bacter... 11975939 - Quantitative in vivo assessment of arginine utilization and nitric oxide production in ... 16442319 - Nitric oxide-induced downregulation of leptin production by 3t3-l1 adipocytes. 12505549 - Regulation of prostaglandin generation in carrageenan-induced pleurisy by inducible nit... 7689839 - Transforming growth factor beta differentially modulates the inducible nitric oxide syn... 18852009 - Carnitine deficiency aggravates carboplatin nephropathy through deterioration of energy... 8483479 - Parathyroid hormone represses alpha 1(i) collagen promoter activity in cultured calvari... 14766969 - Defective cardiovascular development and elevated cyclin e and notch proteins in mice l... 11287649 - Expression of the telomerase catalytic subunit, htert, induces resistance to transformi... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: European journal of immunology Volume: 30 ISSN: 0014-2980 ISO Abbreviation: Eur. J. Immunol. Publication Date: 2000 Sep |
Date Detail:
|
Created Date: 2000-10-12 Completed Date: 2000-10-12 Revised Date: 2007-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 1273201 Medline TA: Eur J Immunol Country: GERMANY |
Other Details:
|
Languages: eng Pagination: 2555-64 Citation Subset: IM |
Affiliation:
|
Centre de Recherche en Infectiologie and Département de Biologie Médicale, Centre Hospitalier Universitaire de Québec, Faculté de Médecine, Université Laval, Ste-Foy, Canada. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cytokines / biosynthesis, genetics Enzyme Inhibitors / pharmacology* Female Leishmaniasis / immunology, prevention & control* Macrophage Activation Mice Mice, Inbred BALB C Mice, Inbred C57BL Neutrophils / immunology Nitric Oxide / physiology* Nitric Oxide Synthase / physiology Nitric Oxide Synthase Type II Protein Tyrosine Phosphatases / antagonists & inhibitors*, physiology RNA, Messenger / analysis Vanadium Compounds / pharmacology* |
| Chemical | |
Reg. No./Substance:
|
0/Cytokines; 0/Enzyme Inhibitors; 0/RNA, Messenger; 0/Vanadium Compounds; 10102-43-9/Nitric Oxide; EC 1.14.13.39/Nitric Oxide Synthase; EC 1.14.13.39/Nitric Oxide Synthase Type II; EC 1.14.13.39/Nos2 protein, mouse; EC 3.1.3.48/Protein Tyrosine Phosphatases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Role of CD154 in the secondary immune response: the reduction of pre-existing splenic germinal cente...
Next Document: Interleukin-12 and Flt3 ligand differentially promote dendropoiesis in vivo.