| Peroxisomes in human and mouse testis: differential expression of peroxisomal proteins in germ cells and distinct somatic cell types of the testis. | |
| | |
MedLine Citation:
|
PMID: 17881773 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The vital importance of peroxisomal metabolism for regular function of the testis is stressed by the severe spermatogenesis defects induced by peroxisomal dysfunction. However, only sparse information is available on the role and enzyme composition of this organelle in distinct cell types of the testis. In the present study, we characterized the peroxisomal compartment in human and mouse testis in primary cultures of murine somatic cells (Sertoli, peritubular myoid, and Leydig cells) and in GFP-PTS1 transgenic mice with a variety of morphological and biochemical techniques. Formerly, peroxisomes were thought to be absent in late stages of spermatogenesis. However, our results obtained by detection of different peroxisomal marker proteins show the presence of these organelles in most cell types in the testis, except for mature spermatozoa. Furthermore, we demonstrate a strong heterogeneity of peroxisomal protein content in various cell types of the human and mouse testis and show marked differences in structure, abundance, and localization of these organelles in spermatids, depending on their maturation. Highest and selective enrichment of the peroxisomal lipid transporters (ABCD1 and ABCD3) as well as ACOX2, the key regulatory enzyme of the beta-oxidation pathway 2 for side chain oxidation of cholesterol, were found in Sertoli cells, whereas Leydig cells were enriched in catalase and ABCD2. Our results suggest a cell type-specific metabolic function of peroxisomes in the testis and point to an important role for peroxisomes in spermiogenesis and in the lipid metabolism of Sertoli cells. |
| | |
Authors:
|
Anca Nenicu; Georg H Lüers; Werner Kovacs; M David; Andreas Zimmer; Martin Bergmann; Eveline Baumgart-Vogt |
Related Documents
:
|
7964303 - Differential effects of the administration of human chorionic gonadotropin to postnatal... 20727243 - Testicular structure and spermatogenesis of amazonian freshwater cururu stingray potamo... 22888233 - Synthesis and application of luminescent single cds quantum dot encapsulated silica nan... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-09-19 |
Journal Detail:
|
Title: Biology of reproduction Volume: 77 ISSN: 0006-3363 ISO Abbreviation: Biol. Reprod. Publication Date: 2007 Dec |
Date Detail:
|
Created Date: 2007-11-22 Completed Date: 2008-04-04 Revised Date: 2008-04-05 |
Medline Journal Info:
|
Nlm Unique ID: 0207224 Medline TA: Biol Reprod Country: United States |
Other Details:
|
Languages: eng Pagination: 1060-72 Citation Subset: IM |
Affiliation:
|
Institute for Anatomy and Cell Biology II, Justus Liebig University, 35385 Giessen, Germany. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Biological Markers / metabolism Humans Lipid Metabolism / physiology* Male Mice Mice, Inbred C57BL Mice, Transgenic Peroxisomes / metabolism, physiology, ultrastructure* Sperm Maturation / physiology* Testis / cytology, metabolism, ultrastructure* |
| Chemical | |
Reg. No./Substance:
|
0/Biological Markers |
| Comments/Corrections | |
Erratum In:
|
Biol Reprod. 2008 Feb;78(2):378 Note: David, M [added]; Zimmer, Andreas [added] |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Developmental changes in extracellular matrix messenger RNAs in the mouse placenta during the second...
Next Document: Modes of actions of two types of anti-neoplastic drugs, dacarbazine and ACNU, to induce apoptosis.