Document Detail


Peroxisome-proliferator-activated receptor delta mediates the effects of long-chain fatty acids on post-confluent cell proliferation.
MedLine Citation:
PMID:  10926831     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Nutritional long-chain fatty acids control adipose tissue mass by regulating the number and the size of adipocytes. It is now established that peroxisome-proliferator-activated receptors (PPARs) play crucial functions in the control of gene expression and the level of cell differentiation. PPARgamma, which is activated by specific prostanoids, is a key factor in activating terminal differentiation and adipogenesis. We have recently demonstrated that PPARdelta, once activated by fatty acids, drives the expression of a limited set of genes, including that encoding PPARgamma, thereby inducing adipose differentiation. Thus far, the mechanism of action of fatty acids in the control of preadipocyte proliferation has remained unknown. We show here that PPARdelta is directly implicated in fatty acid-induced cell proliferation. Ectopic expression of PPARdelta renders 3T3C2 cells capable of responding to treatment with long-chain fatty acids by a resumption of mitosis, and this effect is limited to a few days after confluence. This response is restricted to PPARdelta activators and, for fatty acids, takes place within the range of concentrations found to trigger differentiation of preadipocytes both in vitro and in vivo. Furthermore, the use of a mutated inactive PPARdelta demonstrated that transcriptional activity of the nuclear receptor is required to mediate fatty acid-induced proliferation. These data demonstrate that PPARdelta, as a transcription factor, is directly implicated in fatty acid-induced proliferation, and this could explain the hyperplastic development of adipose tissue that occurs in high-fat-fed animals.
Authors:
C Jehl-Pietri; C Bastie; I Gillot; S Luquet; P A Grimaldi
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Biochemical journal     Volume:  350 Pt 1     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  2000 Aug 
Date Detail:
Created Date:  2001-01-02     Completed Date:  2001-01-11     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  93-8     Citation Subset:  IM    
Affiliation:
Institut National de la Santé et de la Recherche Médicale U470, Centre de Biochimie, Parc Valrose, UFR Sciences, Université de Nice-Sophia Antipolis, 06108 Nice, France.
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MeSH Terms
Descriptor/Qualifier:
3T3 Cells
Animals
Base Sequence
Cell Division / drug effects*
DNA Primers
Fatty Acids / pharmacology*
Mice
Receptors, Cytoplasmic and Nuclear / agonists,  physiology*
Transcription Factors / agonists,  physiology*
Transcription, Genetic / physiology
Chemical
Reg. No./Substance:
0/DNA Primers; 0/Fatty Acids; 0/Receptors, Cytoplasmic and Nuclear; 0/Transcription Factors
Comments/Corrections

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