Document Detail


Peroxisome proliferator-activated receptor γ agonists reduce cell proliferation and viability and increase apoptosis in SSc fibroblasts.
MedLine Citation:
PMID:  22860752     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Background and Objectives -  No study has evaluated the effect of the peroxisome proliferator-activated receptor γ (PPARγ) agonists on cell viability, proliferation and apoptosis in cultured Systemic Sclerosis (SSc) fibroblasts. Methods -  The effects of two pure PPARγ agonists (rosiglitazone and pioglitazone) in cultured SSc fibroblasts were evaluated and compared to normal fibroblasts. The study included the evaluation of the cell viability and proliferation (based on the cleavage of tetrazolium salts and on the measurement of the absorbance of the cell proliferation reagent WST-1), and the cell apoptosis (by means of the Hoechst dye uptake). Results -  Rosiglitazone or pioglitazone (20 μM) significantly reduced cell proliferation (cell counting of 75% and 83% compared to baseline, respectively, after 2 h) and cell viability (absorbance reductions of 25% and 22% compared to baseline, respectively, after 2 h), and increased apoptosis (apoptotic cell percentages 9.9% and 8.6%, respectively, after 48 h of incubation) in SSc fibroblasts, whereas they did not present a significant influence on control fibroblasts. Conclusions -  The effects of rosiglitazone or pioglitazone showed on SSc fibroblasts raise the hypothesis of a therapeutic role for PPARγ agonists in patients affected by SSc.
Authors:
A Antonelli; C Ferri; S M Ferrari; M Colaci; I Ruffilli; M Sebastiani; P Fallahi
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-8-4
Journal Detail:
Title:  The British journal of dermatology     Volume:  -     ISSN:  1365-2133     ISO Abbreviation:  Br. J. Dermatol.     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-8-6     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0004041     Medline TA:  Br J Dermatol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012 British Association of Dermatologists.
Affiliation:
Department of Internal Medicine, Metabolism Unit, University of Pisa School of Medicine, Pisa, Italy. Department of Internal Medicine, Rheumatology Unit, University of Modena & Reggio E. School of Medicine, Modena, Italy.
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