Document Detail


Peroxisomal beta-oxidation from endogenous substrates. Demonstration through H2O2 production in the unanaesthetized mouse.
MedLine Citation:
PMID:  6372785     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A system was developed in which it is possible to detect in vivo changes in hepatic H2O2 production, using a combination of the catalase inhibitor, 3-amino-1,2,4-triazole and methanol. In mice, starvation significantly increases hepatic H2O2 production and plasma non-esterified fatty acid concentrations. Short-term refeeding after a 24 h starvation period brings H2O2 production and plasma non-esterified fatty acid concentration back to normal in 3h. Administration of insulin 24 h after the onset of starvation normalizes H2O2 production in less than 2h and decreases non-esterified fatty acid concentration below normal values. The suppression by insulin of H2O2 production, as well as its coherence with plasma non-esterified fatty acid concentration, indicate that increased H2O2 production in starved mice reflects peroxisomal beta-oxidation.
Authors:
C Van den Branden; I Kerckaert; F Roels
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Biochemical journal     Volume:  218     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1984 Mar 
Date Detail:
Created Date:  1984-06-08     Completed Date:  1984-06-08     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  697-702     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amitrole / pharmacology
Animals
Catalase / metabolism
Fatty Acids, Nonesterified / blood
Hydrogen Peroxide / metabolism*
Insulin / pharmacology
Liver / drug effects,  metabolism*
Male
Methanol / pharmacology
Mice
Mice, Inbred BALB C
Microbodies / drug effects,  metabolism*
Oxidation-Reduction
Starvation / metabolism
Chemical
Reg. No./Substance:
0/Fatty Acids, Nonesterified; 11061-68-0/Insulin; 61-82-5/Amitrole; 67-56-1/Methanol; 7722-84-1/Hydrogen Peroxide; EC 1.11.1.6/Catalase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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