| Peroxiredoxin III protects pancreatic {beta} cells from apoptosis. | |
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MedLine Citation:
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PMID: 20807727 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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Type 1 diabetes mellitus is characterized by a progressive autoimmune destruction of insulin-producing β cells. Macrophages and T lymphocytes release cytokines, which induce the synthesis of oxygen and nitrogen radicals in the pancreatic islets. The resulting cellular and mitochondrial damage promotes β cell death. β cells are very sensitive to the autoimmune free radical-dependent attack due to their low content of antioxidant enzymes such as glutathione peroxidase and catalase. A focal point of β cell protection should be the control of the mitochondrial redox status, which will result in the preservation of metabolic stimulus-secretion coupling. For this reason, there is a considerable interest in the mitochondrial peroxiredoxin III (PRX III), a thioredoxin-dependent peroxide reductase, which was shown to be able to protect against both oxidative and nitrosative stress. Using the Tet-On-system, we generated stably transfected rat insulinoma cells over- or under-expressing PRX III in a doxycyclin-dependent manner to analyze the effect of increased or decreased amounts of cellular PRX III, following treatment with several stressors. We provide evidence that PRX III protects pancreatic β cells from cell stress induced by accumulation of hydrogen peroxide, or the induction of inducible nitric oxide synthase or caspase-9 and -3 by pro-inflammatory cytokines or streptozotocin. Basal insulin secretion was markedly decreased in cells expressing lower levels of PRX III. We suggest PRX III may be a suitable target for promoting deceleration or even prevention of stress-associated apoptosis in pancreatic β cells and the manifestation of insulin-dependent diabetes mellitus. |
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Authors:
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Gabriele Wolf; Nicole Aumann; Marta Michalska; Antje Bast; Jürgen Sonnemann; James F Beck; Uwe Lendeckel; Philip Newsholme; Reinhard Walther |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-08-31 |
Journal Detail:
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Title: The Journal of endocrinology Volume: 207 ISSN: 1479-6805 ISO Abbreviation: J. Endocrinol. Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-10-11 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0375363 Medline TA: J Endocrinol Country: England |
Other Details:
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Languages: eng Pagination: 163-75 Citation Subset: IM |
Affiliation:
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Department of Medical Biochemistry and Molecular Biology, University of Greifswald, Klinikum, Sauerbruchstrasse, D-17487 Greifswald, Germany. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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