| Peroxidase mimicking DNA-gold nanoparticles for fluorescence detection of the lead ions in blood. | |
| | |
MedLine Citation:
|
PMID: 23032966 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Oligonucleotide (T30695) modified gold nanoparticles (T30695-Au NPs) have been prepared and employed for quantification of lead ions (Pb(2+)) in blood. The detection of Pb(2+) ions is through the formation of Au-Pb alloys and oligonucleotide-Pb(2+) complexes that catalyze the H(2)O(2)-mediated oxidation of non-fluorescent Amplex UltraRed (AUR) to form a highly fluorescent oxidized AUR product. Surface-assisted laser desorption/ionization time-of-flight mass spectrometry (SALDI-TOF MS) and inductively coupled plasma mass spectrometry (ICP-MS) revealed the formation of Au-Pb alloys on the surfaces of the 40T30695-Au NPs (i.e., the system featuring 40 molecules of T30695 per Au NP) in the presence of Pb(2+) ions, leading to increased catalytic activity for the H(2)O(2)-mediated oxidation of AUR. The fluorescence intensity (excitation/emission maxima: ca. 540/584 nm) of the oxidized AUR product is proportional to the concentration of Pb(2+) ions over the range 0.1-100 nM, with a linear correlation (R(2) = 0.99). The 40T30695-Au NP/AUR probe is highly selective toward Pb(2+) ions (by at least 200-fold over other tested metal ions). The 40T30695-Au NPs/AUR probe provided limits of detection (LOD, at a signal-to-noise ratio 3) for Pb(2+) ions of 0.05 and 0.1 nM, in Tris-acetate solution (5 mM, pH 8.0) without and with salt (150 mM NaCl, 5 mM KCl, 1 mM MgCl(2), and 1 mM CaCl(2)), respectively. Without conducting tedious sample pretreatment, the approach allows detection of Pb(2+) ions in blood samples, showing the potential of the 40T30695-Au NPs/AUR assay for on-site and real-time detection of Pb(2+) ions in biological samples. |
| | |
Authors:
|
Chi-Lin Li; Chih-Ching Huang; Wei-Hsi Chen; Cheng-Kang Chiang; Huan-Tsung Chang |
Related Documents
:
|
21688786 - Synthesis of azaheterocycles from aryl ketone o-acetyloximes and internal alkynes by cu... 21696206 - Mechanism of n/o bond scission of n2o by an unsaturated rhodium transient. 21592466 - Transformation of aldose formazans. novel synthesis of 2-acetamido-2-deoxypentonolacton... 21902206 - Synthesis of benzothiophene derivatives by pd-catalyzed or radical-promoted heterocyclo... 21814686 - Nickel complexes of a bis(benzimidazolin-2-ylidene)pyridine pincer ligand with four- an... 2583086 - Synthesis and conformational analysis of a series of galactosyl enkephalin analogues sh... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-10-3 |
Journal Detail:
|
Title: The Analyst Volume: - ISSN: 1364-5528 ISO Abbreviation: Analyst Publication Date: 2012 Oct |
Date Detail:
|
Created Date: 2012-10-3 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0372652 Medline TA: Analyst Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
Department of Chemistry, National Taiwan University, 1, Section 4, Roosevelt Road, Taipei, 10617, Taiwan. changht@ntu.edu.tw. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Thermodynamics and Conformations in the Formation of Excited States and Their Interconversions for T...
Next Document: A general route to make non-conjugated linear polymers luminescent.