| Permeation of polysucrose 15000 across the human nasal mucosa in vivo. | |
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MedLine Citation:
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PMID: 9450143 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Studies of the nasal permeation of small molecules (< 1000 Da) have yielded important information about the integrity of the human airway mucosa in health and disease. In this study, we used a much larger tracer molecule, polysucrose (PS) 15,000 (approx. 14,700 Da), to predict the mucosal permeation of inhalational allergens. PS 15,000 (50 mg/ml; 15 ml), with or without a detergent type of permeation enhancer (dioctyl sodium sulfosuccinate 10 mg/ml), was maintained for 15 min in one nasal cavity of 12 healthy nonatopic subjects by employment of a nasal-pool device. Permeation as determined by the 24-h urine recovery of PS (micro-ELISA analysis assay) was expressed as percentage of nasal instillate. Mean baseline permeation was 0.044% (range 0.009-0.250%). In the presence of the detergent, permeation increased to 0.600% (range 0.007-2.260%) (P < 0.01). After oral intake of 750 mg of PS 15,000 (50 micrograms/ml; 15 ml), the 24-h urinary recovery was 0.013% (range 0.004-0.023%). Our study thus demonstrates a measurable baseline permeation of PS 15,000, an elevated permeation rate in the presence of an epithelium-damaging detergent molecule, and a negligible permeation by the oral route. These properties support the utility of PS 15,000 as a nasal airway permeation tracer. Its size further suggests that its permeation may reflect mucosal perviousness to many allergens. |
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Authors:
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M Andersson; L Greiff; H Oman; C Svensson; P Wollmer; C G Persson |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Allergy Volume: 52 ISSN: 0105-4538 ISO Abbreviation: Allergy Publication Date: 1997 Dec |
Date Detail:
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Created Date: 1998-03-12 Completed Date: 1998-03-12 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7804028 Medline TA: Allergy Country: DENMARK |
Other Details:
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Languages: eng Pagination: 1229-32 Citation Subset: IM |
Affiliation:
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Department of Otorhinolaryngology Head and Neck Surgery, University Hospital, Lund, Sweden. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Female Humans Male Molecular Weight Nasal Mucosa / metabolism* Permeability Polymers / pharmacokinetics* Sucrose / pharmacokinetics* |
| Chemical | |
Reg. No./Substance:
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0/Polymers; 0/polysucrose 15000; 57-50-1/Sucrose |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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