Document Detail

Permeation of polysucrose 15000 across the human nasal mucosa in vivo.
MedLine Citation:
PMID:  9450143     Owner:  NLM     Status:  MEDLINE    
Studies of the nasal permeation of small molecules (< 1000 Da) have yielded important information about the integrity of the human airway mucosa in health and disease. In this study, we used a much larger tracer molecule, polysucrose (PS) 15,000 (approx. 14,700 Da), to predict the mucosal permeation of inhalational allergens. PS 15,000 (50 mg/ml; 15 ml), with or without a detergent type of permeation enhancer (dioctyl sodium sulfosuccinate 10 mg/ml), was maintained for 15 min in one nasal cavity of 12 healthy nonatopic subjects by employment of a nasal-pool device. Permeation as determined by the 24-h urine recovery of PS (micro-ELISA analysis assay) was expressed as percentage of nasal instillate. Mean baseline permeation was 0.044% (range 0.009-0.250%). In the presence of the detergent, permeation increased to 0.600% (range 0.007-2.260%) (P < 0.01). After oral intake of 750 mg of PS 15,000 (50 micrograms/ml; 15 ml), the 24-h urinary recovery was 0.013% (range 0.004-0.023%). Our study thus demonstrates a measurable baseline permeation of PS 15,000, an elevated permeation rate in the presence of an epithelium-damaging detergent molecule, and a negligible permeation by the oral route. These properties support the utility of PS 15,000 as a nasal airway permeation tracer. Its size further suggests that its permeation may reflect mucosal perviousness to many allergens.
M Andersson; L Greiff; H Oman; C Svensson; P Wollmer; C G Persson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Allergy     Volume:  52     ISSN:  0105-4538     ISO Abbreviation:  Allergy     Publication Date:  1997 Dec 
Date Detail:
Created Date:  1998-03-12     Completed Date:  1998-03-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7804028     Medline TA:  Allergy     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  1229-32     Citation Subset:  IM    
Department of Otorhinolaryngology Head and Neck Surgery, University Hospital, Lund, Sweden.
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MeSH Terms
Molecular Weight
Nasal Mucosa / metabolism*
Polymers / pharmacokinetics*
Sucrose / pharmacokinetics*
Reg. No./Substance:
0/Polymers; 0/polysucrose 15000; 57-50-1/Sucrose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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