Document Detail


Permeant lipophilicity and vehicle composition influence accumulation of dyes in hair follicles of human skin.
MedLine Citation:
PMID:  12694885     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In skin and hair research drug targeting to the hair follicle is of great interest. Therefore the influence of permeant lipophilicity and vehicle composition on local accumulation has been examined using confocal laser scanning microscopy (CLSM). Formulations saturated with either Oregon Green 488, Bodipy FL C(5) or Bodipy 564/570 C(5) were prepared. The dyes were applied in citric acid buffer, 8% (w/v) surfactants in citric acid buffer or 8% (w/v) surfactants/20% (w/v) propylene glycol in citric acid buffer. Flow-through diffusion experiments were performed with fresh human scalp skin, after which the skin was imaged using CLSM. Diffusion studies showed for Oregon Green 488 (low lipophilicity) a higher flux when applied in citric acid buffer compared to surfactants. In contrast the fluxes of the more lipophilic dyes (Bodipy FL C(5) and Bodipy 564/570 C(5)) are highest when applied in surfactants/propylene glycol. CLSM studies revealed that follicular accumulation increased with (i) a lipophilic dye and (ii) application of lipophilic dyes in surfactants-propylene glycol. Therefore we conclude that targeting to the hair follicle can be increased by the use of lipophilic drugs in combination with surfactant solutions and propylene glycol.
Authors:
Ylva Y Grams; Soile Alaruikka; Lisa Lashley; Julia Caussin; Lynne Whitehead; Joke A Bouwstra
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences     Volume:  18     ISSN:  0928-0987     ISO Abbreviation:  Eur J Pharm Sci     Publication Date:  2003 Apr 
Date Detail:
Created Date:  2003-04-15     Completed Date:  2004-02-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9317982     Medline TA:  Eur J Pharm Sci     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  329-36     Citation Subset:  IM    
Affiliation:
Leiden/Amsterdam Center for Drug Research, Department of Pharmaceutical Technology, Leiden University, P.O. Box 9502, Einsteinweg 55, 2300 RA Leiden, The Netherlands.
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MeSH Terms
Descriptor/Qualifier:
Boron Compounds / pharmacokinetics*
Carboxylic Acids / pharmacokinetics*
Chemistry, Pharmaceutical
Diffusion
Hair Dyes / pharmacokinetics*
Hair Follicle / metabolism*
Humans
Microscopy, Confocal
Propylene Glycol / chemistry,  pharmacology
Scalp
Skin Absorption / drug effects
Surface-Active Agents / chemistry,  pharmacology
Tissue Distribution
Vehicles / chemistry,  pharmacology*
Chemical
Reg. No./Substance:
0/4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; 0/Boron Compounds; 0/Carboxylic Acids; 0/Hair Dyes; 0/Oregon Green 488 carboxylic acid; 0/Surface-Active Agents; 0/Vehicles; 57-55-6/Propylene Glycol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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