Document Detail

Perivascular treatment with azathioprine reduces neointimal hyperplasia in experimental vein grafts.
MedLine Citation:
PMID:  16401538     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Azathioprine is an immunosuppressive and anti-inflammatory drug, and it has been shown to induce apoptosis in human T-lymphocytes. We investigated whether local treatment with azathioprine can inhibit neointimal hyperplasia in experimental vein grafts. METHODS: C57BL/6J mice underwent interposition of the inferior vena cava from isogenic donor mice into the common carotid artery using a cuff technique. In the treatment group azathioprine was perivascularly applied. The control group did not receive local treatment. Vein grafts were harvested at 1 and 2 weeks postoperatively and underwent morphometric analysis as well as immunohistochemical analysis for apoptosis (TUNEL). RESULTS: In grafted veins without treatment (controls), neointimal thickness was 10 microm (range, 6-29 microm), and 12 microm (range, 8-40 microm) at 1 and 2 weeks postoperatively, respectively. In azathioprine-treated grafts, the neointimal thickness was 2 microm (range, 1-5 microm) and 4 microm (range, 3-11 microm) at 1 and 2 weeks postoperatively, respectively. This reduction of neointimal thickness was significant at 1 week (P = .001) and 2 weeks (P = .016) postoperatively. Azathioprine-treated vein grafts showed an increased rate of apoptosis in the vascular wall as compared with controls (593 [range, 26-783] versus 45 [range, 0-106] apoptotic cells/mm(2) at 1 week, P = .063; and 656 [range, 327-1270] versus 19 [range, 0-79] apoptotic cells/mm(2) at 2 weeks, P = .016). CONCLUSION: We conclude that treatment of experimental vein grafts with azathioprine is associated with a reduction of neointimal hyperplasia and an increased apoptosis rate in the vascular wall. These results suggest that azathioprine may be useful for the prevention of vein graft disease after coronary artery bypass grafting.
Thomas Schachner; Simone Heiss; Daniel Zipponi; Alexandar Tzankov; Oliver Bernecker; Arthur Kroell; Günther Laufer; Johannes Bonatti
Related Documents :
7904148 - Effect of chronic native flow competition on internal thoracic artery grafts.
6448828 - Perigraft lipid accumulation with unusual complications in a hyperlipidemic patient.
21044158 - The patency of sequential and individual saphenous vein grafts after off-pump coronary ...
2946748 - The pathogenesis and prevention of aortocoronary vein bypass graft occlusion and resten...
18049858 - Bronchopulmonary foregut malformation in an adult.
21135488 - Diagnosis, therapy monitoring and follow up of renal artery pseudoaneurysm with contras...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The heart surgery forum     Volume:  9     ISSN:  1522-6662     ISO Abbreviation:  Heart Surg Forum     Publication Date:  2006  
Date Detail:
Created Date:  2006-01-10     Completed Date:  2006-12-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100891112     Medline TA:  Heart Surg Forum     Country:  United States    
Other Details:
Languages:  eng     Pagination:  E515-7     Citation Subset:  IM    
Department of Cardiac Surgery, Innsbruck Medical University, Innsbruck, Austria.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Azathioprine / pharmacology*
Carotid Artery, Common / surgery
Immunosuppressive Agents / pharmacology*
In Situ Nick-End Labeling
Mice, Inbred C57BL
Tunica Intima / drug effects*,  pathology*
Vascular Surgical Procedures
Vena Cava, Inferior / pathology,  transplantation*
Reg. No./Substance:
0/Immunosuppressive Agents; 446-86-6/Azathioprine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Minimally invasive vein harvesting with the SaphLITE retractor system: is it really better?
Next Document:  Quality of anastomosis in conventional on-pump coronary artery bypass graft surgery: influence of th...