Document Detail


Periplasmic oxygen reduction by Desulfovibrio species.
MedLine Citation:
PMID:  11685376     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Washed cells of Desulfovibrio vulgaris strain Marburg (DSM 2119) reduced oxygen to water with H(2) as electron donor at a mean rate of 253 nmol O(2) min(-1) (mg protein)(-1). After separating the periplasm from the cells, more than 60% of the cytochrome c activity and 90% of the oxygen-reducing activity were found in the periplasmic fraction. Oxygen reduction and the reduction of cytochrome c with H(2) were inhibited by CuCl(2). After further separation of the periplasm by ultrafiltration (exclusion sizes 30, 50, and 100 kDa), oxygen reduction with H(2) occurred with the retentates only. Ascorbate plus tetramethyl-p-phenylenediamine (TMPD), however, were also oxidized by the filtrates. The stoichiometry of 1 mol O(2) reduced per 2 mol ascorbate oxidized indicated the formation of water. Our experiments present evidence that in D. vulgaris periplasmic hydrogenase and cytochrome c play a major role in oxygen reduction. Preliminary studies with other Desulfovibrio species indicated a similar function of periplasmic c-type cytochromes in D. desulfuricans CSN and D. termitidis KH1.
Authors:
A Baumgarten; I Redenius; J Kranczoch; H Cypionka
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Archives of microbiology     Volume:  176     ISSN:  0302-8933     ISO Abbreviation:  Arch. Microbiol.     Publication Date:  2001 Oct 
Date Detail:
Created Date:  2001-10-30     Completed Date:  2001-12-07     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0410427     Medline TA:  Arch Microbiol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  306-9     Citation Subset:  IM    
Affiliation:
Institut für Chemie und Biologie des Meeres, Universität Oldenburg, 26111 Oldenburg, Germany.
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MeSH Terms
Descriptor/Qualifier:
Desulfovibrio vulgaris / enzymology,  growth & development,  metabolism*
Hydrogen / metabolism
Hydrogenase / chemistry,  metabolism
Oxidation-Reduction
Oxygen / metabolism*
Periplasm / metabolism
Sulfur Compounds / metabolism
Chemical
Reg. No./Substance:
0/Sulfur Compounds; 1333-74-0/Hydrogen; 7782-44-7/Oxygen; EC 1.12.7.2/Hydrogenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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