Document Detail


Peripheral mu-opioid receptors attenuate the augmented exercise pressor reflex in rats with chronic femoral artery occlusion.
MedLine Citation:
PMID:  20543079     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Recently, opioid receptors have been shown to be expressed on group III and IV afferents, which comprise the sensory arm of the exercise pressor reflex. Although the stimulation of opioid receptors in the central nervous system has been shown to attenuate the exercise pressor reflex, the effect on the reflex of their stimulation in the periphery is unknown. We therefore tested the hypothesis that the activation of peripheral mu-opioid receptors attenuates the exercise pressor reflex. The pressor responses to static contraction were compared before and after the injection of the mu-opioid receptor agonist [d-Ala(2),N-MePhe(4),Gly-ol(5)]enkephalin (DAMGO; 1 microg) into the abdominal aorta of decerebrated rats in which one femoral artery had been occluded 72 h previously (n = 10) and in control rats whose femoral arteries were freely perfused (n = 8). DAMGO attenuated the peak pressor response to contraction in rats whose femoral arteries had been occluded (before: increase of 34 + or - 3 mmHg and after: increase of 22 + or - 2 mmHg, P = 0.008); the inhibitory effect of DAMGO was prevented by the injection of naloxone (100 microg) into the abdominal aorta (before: increase of 29 + or - 5 mmHg and after: increase of 29 + or - 5 mmHg, P = 0.646, n = 7). An intravenous injection of DAMGO (1 microg, n = 6) had no effect on the peak pressor response to contraction in both groups of rats. DAMGO had no effect on the peak pressor response to contraction in rats whose femoral arteries were freely perfused (before: Delta 23 + or - 4 mmHg, after: Delta 23 + or - 3 mmHg, n = 6) but appeared to have a small effect on topography of the response. DAMGO had no effect on the peak pressor response to tendon stretch in both groups of rats (both P > 0.05). We conclude that the stimulation of peripheral mu-opioid receptors attenuates the exercise pressor reflex in rats whose femoral arteries have been ligated for 72 h.
Authors:
Hirotsugu Tsuchimochi; Jennifer L McCord; Marc P Kaufman
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2010-06-11
Journal Detail:
Title:  American journal of physiology. Heart and circulatory physiology     Volume:  299     ISSN:  1522-1539     ISO Abbreviation:  Am. J. Physiol. Heart Circ. Physiol.     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-07-30     Completed Date:  2010-08-30     Revised Date:  2011-08-03    
Medline Journal Info:
Nlm Unique ID:  100901228     Medline TA:  Am J Physiol Heart Circ Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  H557-65     Citation Subset:  IM    
Affiliation:
Penn State Heart and Vascular Institute, 500 University Dr., Mail Code H047, Hershey Medical Center, Hershey, PA 17033, USA. htsuchimochi@hmc.psu.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Arterial Occlusive Diseases / metabolism*,  physiopathology
Baroreflex* / drug effects
Chronic Disease
Constriction, Pathologic
Disease Models, Animal
Enkephalin, Ala(2)-MePhe(4)-Gly(5)- / administration & dosage
Femoral Artery / innervation*,  surgery
Hindlimb
Injections, Intra-Arterial
Injections, Intravenous
Ligation
Male
Muscle Contraction
Muscle, Skeletal / blood supply*,  innervation*
Naloxone / administration & dosage
Narcotic Antagonists / administration & dosage
Neurons, Afferent / drug effects,  metabolism
Physical Exertion*
Rats
Rats, Sprague-Dawley
Receptors, Opioid, mu / antagonists & inhibitors,  metabolism*
Time Factors
Grant Support
ID/Acronym/Agency:
R01-AR-059397/AR/NIAMS NIH HHS
Chemical
Reg. No./Substance:
0/Narcotic Antagonists; 0/Receptors, Opioid, mu; 100929-53-1/Enkephalin, Ala(2)-MePhe(4)-Gly(5)-; 465-65-6/Naloxone
Comments/Corrections

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