| Peripheral blood neutrophil function and lymphocyte subpopulations in cycling mares. | |
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MedLine Citation:
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PMID: 14629669 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The purpose of this study was to evaluate different parameters of the immune status in the mare, during the follicular and the luteal phases of the oestrous cycle, in two consecutive years. Functional competence of peripheral blood neutrophils, such as chemotaxis, phagocytosis and oxidative burst was assessed under physiological cyclic conditions (Exp. I). In the second year of this study (Exp. II), besides peripheral blood neutrophil phagocytosis and oxidative burst analysis, circulating lymphocyte subsets were also characterized. The reproductive status in a total of 17 adult cycling mares was evaluated by ultrasonography and further confirmed by plasma progesterone levels. Chemotaxis tests were performed using porous membranes inserted in transwell chambers. Lipopolysaccharide (LPS) from Escherichia coli and N-formyl-Met-Leu-Phe (fMLP) were used as chemoattractants. Measurement of phagocytosis and oxidative burst in blood neutrophils were assessed by flow cytometry using commercially available kits. Quantification of T-lymphocyte subsets was assessed by indirect immunofluorescence staining after incubation with monoclonal antibodies specific for CD2, CD3, CD4 and CD8 cell markers by flow cytometry. Natural killer cells and B cells were estimated mathematically. No significant difference was found in migration, phagocytosis and oxidative burst at either phase of the oestrous cycle. Statistical analysis of total white blood cell counts also showed no significant difference between either phase of the oestrous cycle, although there was a tendency for blood neutrophils to increase in number under the progesterone influence (p = 0.09). Lymphocytic subpopulations did not differ throughout the oestrous cycle. Overall, our results suggest that luteal and follicular phases in cycling mares may not influence the immune status of the mare. |
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Authors:
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R P Roberto Da Costa; H Carvalho; R Agrícola; J Alpoim-Moreira; C Martins; G Ferreira-Dias |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Reproduction in domestic animals = Zuchthygiene Volume: 38 ISSN: 0936-6768 ISO Abbreviation: Reprod. Domest. Anim. Publication Date: 2003 Dec |
Date Detail:
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Created Date: 2003-11-21 Completed Date: 2004-01-29 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9015668 Medline TA: Reprod Domest Anim Country: Germany |
Other Details:
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Languages: eng Pagination: 464-9 Citation Subset: IM |
Affiliation:
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Escola Superior Agrária de Coimbra, 3040 Bencanta, Coimbra, Portugal. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Chemotaxis Estrus / blood, physiology* Female Flow Cytometry Horses / physiology* Lipopolysaccharides / diagnostic use Lymphocyte Subsets / physiology* Neutrophils / physiology* Phagocytosis Respiratory Burst |
| Chemical | |
Reg. No./Substance:
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0/Lipopolysaccharides |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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