| Peripheral blood lymphocytes immunophenotype and serum concentration of soluble HLA class I in burn patients. | |
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MedLine Citation:
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PMID: 12615457 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The level of the soluble form of histocompatibility class I antigens, associated with beta(2)-microglobulin (sHLA-I) has been determined by an ELISA sandwich method in serum from burned patients (n=42) and healthy volunteers (n=30). The sHLA-I level was insignificantly increased in burn patients at the stage of burn shock (1284+/-324U/ml, mean+/-S.E.M.) and after day 28 postburn (1368+/-258U/ml) compared to volunteers (1150+/-90U/ml). At the same time a decrease of sHLA-I levels between 4 and 14 days (638+/-178U/ml) was determined (P<0.05). Increased levels of sHLA, though not significant, were detected in patients with TBSAB >70% in comparison to patients with TBSAB from 30 to 70% during burn shock (1493+/-528 and 1075+/-339U/ml, respectively). Expression of membranous HLA class I antigens (mHLA-I) in peripheral blood lymphocytes (PBLs) was assayed simultaneously by indirect immunofluorescence. The number of CD3(+), CD4(+), CD8(+), CD25(+), CD71(+) and CD26(+) lymphocytes was also evaluated. The expression of mHLA-I in PBLs was increased significantly in patients with TBSAB <70% at early postburn period. Daily monitoring showed that the relative numbers of CD25(+) and CD71(+) lymphocytes in patients varied greatly within short intervals of time during burn shock. The data obtained suggest that mHLA-I expression can reflect postburn lymphocyte activation. The serum content of sHLA-I does not depend on lymphocyte number or activated lymphocyte number in peripheral blood at burned patients. |
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Authors:
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M Ju Lebedev; M A Krizhanova; S A Vilkov; M N Sholkina; E S Vyasmina; A Ju Baryshnikov; V V Novikov |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Burns : journal of the International Society for Burn Injuries Volume: 29 ISSN: 0305-4179 ISO Abbreviation: Burns Publication Date: 2003 Mar |
Date Detail:
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Created Date: 2003-03-04 Completed Date: 2003-04-28 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 8913178 Medline TA: Burns Country: England |
Other Details:
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Languages: eng Pagination: 123-8 Citation Subset: IM |
Affiliation:
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Laboratory of Immunology, Institute of Traumatology and Orthopaedics, 603155 Verhne Volzhskaya nab. 18, Nizhny Novgorod, Russia. miklgito@mail.ru |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adolescent Adult Aged Aged, 80 and over Antigens, CD / blood* Burns / blood, immunology* Enzyme-Linked Immunosorbent Assay Female Histocompatibility Antigens Class I / blood*, immunology Humans Immunophenotyping Lymphocytes / immunology Male Middle Aged Receptors, Antigen / blood T-Lymphocyte Subsets / immunology beta 2-Microglobulin / blood* |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD; 0/Histocompatibility Antigens Class I; 0/Receptors, Antigen; 0/beta 2-Microglobulin |
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