Document Detail


Peripheral blood lymphocytes immunophenotype and serum concentration of soluble HLA class I in burn patients.
MedLine Citation:
PMID:  12615457     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The level of the soluble form of histocompatibility class I antigens, associated with beta(2)-microglobulin (sHLA-I) has been determined by an ELISA sandwich method in serum from burned patients (n=42) and healthy volunteers (n=30). The sHLA-I level was insignificantly increased in burn patients at the stage of burn shock (1284+/-324U/ml, mean+/-S.E.M.) and after day 28 postburn (1368+/-258U/ml) compared to volunteers (1150+/-90U/ml). At the same time a decrease of sHLA-I levels between 4 and 14 days (638+/-178U/ml) was determined (P<0.05). Increased levels of sHLA, though not significant, were detected in patients with TBSAB >70% in comparison to patients with TBSAB from 30 to 70% during burn shock (1493+/-528 and 1075+/-339U/ml, respectively). Expression of membranous HLA class I antigens (mHLA-I) in peripheral blood lymphocytes (PBLs) was assayed simultaneously by indirect immunofluorescence. The number of CD3(+), CD4(+), CD8(+), CD25(+), CD71(+) and CD26(+) lymphocytes was also evaluated. The expression of mHLA-I in PBLs was increased significantly in patients with TBSAB <70% at early postburn period. Daily monitoring showed that the relative numbers of CD25(+) and CD71(+) lymphocytes in patients varied greatly within short intervals of time during burn shock. The data obtained suggest that mHLA-I expression can reflect postburn lymphocyte activation. The serum content of sHLA-I does not depend on lymphocyte number or activated lymphocyte number in peripheral blood at burned patients.
Authors:
M Ju Lebedev; M A Krizhanova; S A Vilkov; M N Sholkina; E S Vyasmina; A Ju Baryshnikov; V V Novikov
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Burns : journal of the International Society for Burn Injuries     Volume:  29     ISSN:  0305-4179     ISO Abbreviation:  Burns     Publication Date:  2003 Mar 
Date Detail:
Created Date:  2003-03-04     Completed Date:  2003-04-28     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8913178     Medline TA:  Burns     Country:  England    
Other Details:
Languages:  eng     Pagination:  123-8     Citation Subset:  IM    
Affiliation:
Laboratory of Immunology, Institute of Traumatology and Orthopaedics, 603155 Verhne Volzhskaya nab. 18, Nizhny Novgorod, Russia. miklgito@mail.ru
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Aged
Aged, 80 and over
Antigens, CD / blood*
Burns / blood,  immunology*
Enzyme-Linked Immunosorbent Assay
Female
Histocompatibility Antigens Class I / blood*,  immunology
Humans
Immunophenotyping
Lymphocytes / immunology
Male
Middle Aged
Receptors, Antigen / blood
T-Lymphocyte Subsets / immunology
beta 2-Microglobulin / blood*
Chemical
Reg. No./Substance:
0/Antigens, CD; 0/Histocompatibility Antigens Class I; 0/Receptors, Antigen; 0/beta 2-Microglobulin

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