Document Detail


Peripheral blood lymphocyte subset shifts in patients with untreated hematological tumors: evidence for systemic activation of the T cell compartment.
MedLine Citation:
PMID:  9593474     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Flow cytometry immunophenotyping of peripheral blood lymphocyte subsets and multivariate data-analytical techniques revealed that among untreated hemato-oncological patients (n = 48) with lymphomas, acute and chronic myeloid and lymphocytic leukemias, monoclonal gammopathy of undetermined significance, and multiple myeloma, 42% had (nonmalignant) lymphocyte profiles clearly distinct from healthy donors. Notably, a similar pattern of increased CD3+ CD57+, CD3+ HLA-DR+, CD3+ CD(16 + 56)+, CD4- CD8+, CD8+ CD57+, CD8+ CD28-, and CD8+ CD62L- subsets was detected. More extensive three-color immunophenotyping on an additional group of 49 untreated patients revealed that both CD4+ and CD8+ T cells displayed significant increases of activation markers: CD69, CD(16 + 56), HLA-DR, CD71, and CD57, and a loss of CD62L and CD28, which is also interpreted as a sign of activation. Consistent with the phenotypical signs of in vivo immune activation, polyclonal cytolytic activity, measured ex vivo in an anti-CD3-redirected assay, was detected within immunomagnetically purified CD4+ T cells of three out of six B-CLL patients investigated, but not within purified CD4+ T cells of five healthy donors. The purified CD8+ T cells of patients (n = 28) and donors (n = 5) on the other hand displayed similar polyclonal cytotoxic activities at the various effector:target ratios investigated. Tumor-directed cytotoxic activity of purified CD4+ (n = 6) and/or CD8+ T cells (n = 15) against freshly isolated autologous tumor cells was not detected in any of the experiments. Collectively, our results demonstrate systemic T cell activation as a common feature in hematological neoplasia, and a markedly enhanced cytolytic activity of the CD4- subset in CLL patients. The reason(s) for this expansion of activated T cells and its pathophysiologic significance, however, remain unclear.
Authors:
L E Van den Hove; P Vandenberghe; S W Van Gool; J L Ceuppens; H Demuynck; G E Verhoef; M A Boogaerts
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Leukemia research     Volume:  22     ISSN:  0145-2126     ISO Abbreviation:  Leuk. Res.     Publication Date:  1998 Feb 
Date Detail:
Created Date:  1998-06-03     Completed Date:  1998-06-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7706787     Medline TA:  Leuk Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  175-84     Citation Subset:  IM    
Affiliation:
Laboratory of Experimental Hematology, Faculty of Medicine, Catholic University of Leuven, Belgium.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Aged
Aged, 80 and over
Antigens, CD / immunology
Hematologic Neoplasms / blood*,  immunology*,  pathology
Humans
Immunophenotyping
Lymphocyte Activation*
Lymphocyte Subsets / immunology*
Middle Aged
T-Lymphocyte Subsets / immunology*
Chemical
Reg. No./Substance:
0/Antigens, CD

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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