Document Detail


Peripheral T cell cytokine responses for diagnosis of active tuberculosis.
MedLine Citation:
PMID:  22523581     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: A test for diagnosis of active Tuberculosis (TB) from peripheral blood could tremendously improve clinical management of patients.
METHODS: Of 178 prospectively enrolled patients with possible TB, 60 patients were diagnosed with pulmonary and 27 patients with extrapulmonary TB. The frequencies of Mycobacterium tuberculosis (MTB) specific CD4(+) T cells and CD8(+) T cells producing cytokines were assessed using overnight stimulation with purified protein derivate (PPD) or early secretory antigenic target (ESAT)-6, respectively.
RESULTS: Among patients with active TB, an increased type 1 cytokine profile consisting of mainly CD4(+) T cell derived interferon (IFN)-γ was detectable. Despite contributing to the cytokine profile as a whole, the independent diagnostic performance of one cytokine producing T cells as well as polyfunctional T cells was poor. IFN-γ/Interleukin(IL)-2 cytokine ratios discriminated best between active TB and other diseases.
CONCLUSION: T cells producing one cytokine and polyfunctional T cells have a limited role in diagnosis of active TB. The significant shift from a "memory type" to an "effector type" cytokine profile may be useful for further development of a rapid immune-diagnostic tool for active TB.
Authors:
Johannes Nemeth; Heide-Maria Winkler; Ralph H Zwick; Catharina Müller; Rudolf Rumetshofer; Lucas Boeck; Otto C Burghuber; Stefan Winkler
Publication Detail:
Type:  Clinical Trial; Journal Article     Date:  2012-04-16
Journal Detail:
Title:  PloS one     Volume:  7     ISSN:  1932-6203     ISO Abbreviation:  PLoS ONE     Publication Date:  2012  
Date Detail:
Created Date:  2012-04-23     Completed Date:  2012-08-03     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  101285081     Medline TA:  PLoS One     Country:  United States    
Other Details:
Languages:  eng     Pagination:  e35290     Citation Subset:  IM    
Affiliation:
Department of Internal Medicine I, Division of Infectious Diseases and Tropical Medicine, Medical University of Vienna, Vienna, Austria.
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Aged
Aged, 80 and over
Antigens, Bacterial / diagnostic use
Bacterial Proteins / diagnostic use
CD4-Positive T-Lymphocytes / immunology
CD8-Positive T-Lymphocytes / immunology
Cytokines / immunology
Female
Humans
Interferon-gamma / diagnostic use
Interleukin-2 / diagnostic use
Male
Middle Aged
Mycobacterium tuberculosis / immunology
T-Lymphocytes / immunology*
Tuberculin Test
Tuberculosis / diagnosis*
Chemical
Reg. No./Substance:
0/Antigens, Bacterial; 0/Bacterial Proteins; 0/Cytokines; 0/ESAT-6 protein, Mycobacterium tuberculosis; 0/Interleukin-2; 82115-62-6/Interferon-gamma
Comments/Corrections

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