Document Detail


Periostin and osteopontin are overexpressed in chronically inflamed sinuses.
MedLine Citation:
PMID:  22287326     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
OBJECTIVE: In chronic rhinosinusitis (CRS), the inflammation leads to a proliferative response in the extracellular matrix (ECM). Periostin and osteopontin are 2 ECM proteins which have received attention for their roles in tissue remodeling in inflammatory diseases of the upper and lower airways. Transforming growth factor beta-1 (TGFβ1) is an inflammatory cytokine that has been implicated in fibrotic conditions affecting virtually every organ. In this study we seek to evaluate the differential expression of periostin, osteopontin, and TGFβ1 in the ethmoid sinus and nasal floor of patients with CRS. Furthermore, we seek to determine if a correlation exists between their differential expression in the nose and sinuses of patients with CRS.
METHODS: Biopsies from ethmoid sinus and nasal floor mucosa were taken from 15 patients undergoing functional endoscopic sinus surgery (FESS) for CRS. Complementary DNA (cDNA) microarray analysis demonstrated upregulation of periostin and osteopontin in the ethmoid sinus samples. Quantitative real-time polymerase chain reaction (RT-PCR) was performed for periostin, osteopontin, and TGFβ1. Statistical analysis was undertaken to examine correlations between the 3 genes of interest.
RESULTS: RT-PCR confirmed that periostin and osteopontin were overexpressed in the ethmoid samples compared to the nasal floor. The differential expression of both periostin and osteopontin showed significant correlation with TGFβ1.
CONCLUSION: Periostin and osteopontin appear to be involved in ECM remodeling seen in CRS. TGFβ1 may be an upstream inducer of CRS-related changes in ethmoid sinus mucosa. © 2011 ARS-AAOA, LLC.
Authors:
Steven Marc Daines; Yong Wang; Richard R Orlandi
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  International forum of allergy & rhinology     Volume:  1     ISSN:  2042-6984     ISO Abbreviation:  Int Forum Allergy Rhinol     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2012-01-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101550261     Medline TA:  Int Forum Allergy Rhinol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  101-5     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 American Rhinologic Society-American Academy of Otolaryngic Allergy, LLC.
Affiliation:
University of Utah School of Medicine, Division of Otolaryngology-Head & Neck Surgery, Salt Lake City, UT. steven.daines@hsc.utah.edu.
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