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Periodontal Disease Decreases Insulin Sensitivity and Insulin Signaling.
MedLine Citation:
PMID:  22087804     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Background: The purpose of this study was to investigate whether local inflammatory events, such as periodontal disease, are able to increase TNF-? plasmatic concentration, and decrease insulin sensitivity and insulin signaling in non-diabetic rats. Methods: Forty-eight male Wistar rats (at two months old) were divided into two groups, with either ligature-induced periodontal disease (PD) or control conditions (CN). Experiments were carried out in both groups twenty-eight days following ligature placement. Plasmatic concentration of glycemia and TNF (n=10) were analyzed by the glucose oxidase and ELISA method, respectively. Insulin sensitivity (n=7) were measured using the insulin tolerance test (ITT). Insulin signal transduction (n=7) was measured by pp185 tyrosine phosphorylation status in insulin-sensitive tissues using the western blotting method. Results: The PD group showed decreased insulin sensitivity (p<0.05), although no glycemic alterations were noted (p>0.05). TNF plasmatic concentration was higher in PD rats compared to control. In addition, a decrease in the pp185 tyrosine phosphorylation status was observed following insulin stimulus in both white adipose and skeletal muscle tissues of the PD group compared to the control group. Conclusions: Periodontal disease is able to cause alterations to both insulin signaling and insulin sensitivity, probably due to the elevation of TNF-? plasmatic concentration. Thus, the present results emphasize the importance of the prevention of local inflammatory diseases, such as periodontitis, to prevent diabetes mellitus.
Authors:
Natalia H Colombo; Daisy J Shirakashi; Fernando Y Chiba; Maria Sara de Lima Coutinho; Edilson Ervolino; Cl A Adas Saliba Garbin; Ubiratan Fabres Machado; Doris H Sumida
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-11-16
Journal Detail:
Title:  Journal of periodontology     Volume:  -     ISSN:  1943-3670     ISO Abbreviation:  -     Publication Date:  2011 Nov 
Date Detail:
Created Date:  2011-11-17     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8000345     Medline TA:  J Periodontol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Department of Basic Sciences, Dental School of Ara?atuba, S?o Paulo State University -UNESP, Brazil.
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