Document Detail

Periodic mitotic events induced in the absence of DNA replication.
MedLine Citation:
PMID:  3480528     Owner:  NLM     Status:  MEDLINE    
We have discovered and report here a means of separating a mitotic "subcycle" from the G1- and S-phase events of the mammalian cell cycle. Time-lapse videomicroscopy of Syrian hamster fibroblast (BHK) cells revealed that caffeine could induce multiple entries into mitosis while cells were blocked in DNA synthesis. As with normal mitoses, the abundance of mitosis-specific phosphoproteins was coupled with the condensation of chromatin. The BHK temperature-sensitive mutant tsBN2 also completed multiple entries into mitosis while arrested during DNA replication and raised to the restrictive temperature. Periodic mitotic events occurred even when BHK cells were exposed to low concentrations of serum or cycloheximide, conditions that prevent the cycling of BHK cells by blocking their entry into S phase. These results suggest that an oscillation governing the activation and inactivation of mitotic factors can be generated in mammalian cells and uncoupled from the G1 and DNA replication events of the normal cell cycle. This system will be useful for examining the molecular nature of mitotic factors.
R Schlegel; A B Pardee
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  84     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1987 Dec 
Date Detail:
Created Date:  1988-02-20     Completed Date:  1988-02-20     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  9025-9     Citation Subset:  IM    
Department of Pharmacology, Harvard Medical School, Boston, MA 02115.
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MeSH Terms
Caffeine / pharmacology
Cell Cycle*
Cell Division / drug effects
Cell Line
Chromatin / ultrastructure
Cycloheximide / pharmacology
DNA Replication*
Hydroxyurea / pharmacology
Phosphoproteins / metabolism
Grant Support
Reg. No./Substance:
0/Chromatin; 0/Phosphoproteins; 127-07-1/Hydroxyurea; 58-08-2/Caffeine; 66-81-9/Cycloheximide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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