| Periodic mitotic events induced in the absence of DNA replication. | |
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MedLine Citation:
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PMID: 3480528 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have discovered and report here a means of separating a mitotic "subcycle" from the G1- and S-phase events of the mammalian cell cycle. Time-lapse videomicroscopy of Syrian hamster fibroblast (BHK) cells revealed that caffeine could induce multiple entries into mitosis while cells were blocked in DNA synthesis. As with normal mitoses, the abundance of mitosis-specific phosphoproteins was coupled with the condensation of chromatin. The BHK temperature-sensitive mutant tsBN2 also completed multiple entries into mitosis while arrested during DNA replication and raised to the restrictive temperature. Periodic mitotic events occurred even when BHK cells were exposed to low concentrations of serum or cycloheximide, conditions that prevent the cycling of BHK cells by blocking their entry into S phase. These results suggest that an oscillation governing the activation and inactivation of mitotic factors can be generated in mammalian cells and uncoupled from the G1 and DNA replication events of the normal cell cycle. This system will be useful for examining the molecular nature of mitotic factors. |
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Authors:
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R Schlegel; A B Pardee |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 84 ISSN: 0027-8424 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 1987 Dec |
Date Detail:
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Created Date: 1988-02-20 Completed Date: 1988-02-20 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 9025-9 Citation Subset: IM |
Affiliation:
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Department of Pharmacology, Harvard Medical School, Boston, MA 02115. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Caffeine / pharmacology Cell Cycle* Cell Division / drug effects Cell Line Chromatin / ultrastructure Cricetinae Cycloheximide / pharmacology DNA Replication* Hydroxyurea / pharmacology Mitosis* Periodicity Phosphoproteins / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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CA 22427/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Chromatin; 0/Phosphoproteins; 127-07-1/Hydroxyurea; 58-08-2/Caffeine; 66-81-9/Cycloheximide |
| Comments/Corrections | |
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