Document Detail


Periodic cyclin-Cdk activity entrains an autonomous Cdc14 release oscillator.
MedLine Citation:
PMID:  20403323     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
One oscillation of Cyclin-dependent kinase (Cdk) activity, largely driven by periodic synthesis and destruction of cyclins, is tightly coupled to a single complete eukaryotic cell division cycle. Tight linkage of different steps in diverse cell-cycle processes to Cdk activity has been proposed to explain this coupling. Here, we demonstrate an intrinsically oscillatory module controlling nucleolar release and resequestration of the Cdc14 phosphatase, which is essential for mitotic exit in budding yeast. We find that this Cdc14 release oscillator functions at constant and physiological cyclin-Cdk levels, and is therefore independent of the Cdk oscillator. However, the frequency of the release oscillator is regulated by cyclin-Cdk activity. This observation together with its mechanism suggests that the intrinsically autonomous Cdc14 release cycles are locked at once-per-cell-cycle through entrainment by the Cdk oscillator in wild-type cells. This concept may have broad implications for the structure and evolution of eukaryotic cell-cycle control.
Authors:
Ying Lu; Frederick R Cross
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Cell     Volume:  141     ISSN:  1097-4172     ISO Abbreviation:  Cell     Publication Date:  2010 Apr 
Date Detail:
Created Date:  2010-04-20     Completed Date:  2010-05-04     Revised Date:  2012-06-12    
Medline Journal Info:
Nlm Unique ID:  0413066     Medline TA:  Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  268-79     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier Inc. All rights reserved.
Affiliation:
The Rockefeller University, 1230 York Avenue, New York, New York 10065, USA.
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MeSH Terms
Descriptor/Qualifier:
Cell Cycle Proteins / metabolism*
Cell Nucleolus / metabolism
Cyclin B / metabolism
Cyclins / metabolism*
Mitosis
Models, Biological
Protein Kinases / metabolism
Protein Tyrosine Phosphatases / metabolism*
Saccharomyces cerevisiae / cytology*,  metabolism*
Saccharomyces cerevisiae Proteins / metabolism*
Grant Support
ID/Acronym/Agency:
GM078153/GM/NIGMS NIH HHS; GM47238/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/CDC14 protein, S cerevisiae; 0/CLB2 protein, S cerevisiae; 0/Cell Cycle Proteins; 0/Cyclin B; 0/Cyclins; 0/Hct1 protein, S cerevisiae; 0/Saccharomyces cerevisiae Proteins; EC 2.7.-/Protein Kinases; EC 2.7.11.21/CDC5 protein, S cerevisiae; EC 3.1.3.48/Protein Tyrosine Phosphatases
Comments/Corrections
Comment In:
Cell. 2010 Apr 16;141(2):224-6   [PMID:  20403319 ]

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