Document Detail


Periodic acceleration (pGz) prior to whole body ischemia reperfusion injury provides early cardioprotective preconditioning.
MedLine Citation:
PMID:  20211190     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIMS: Periodic acceleration (pGz) is a method that applies repetitive sinusoidal head-to-foot motion to the horizontally positioned body. pGz adds pulses to the circulation as a function of frequency, thereby increasing shear stress to the endothelium. Pulsatile shear stress increases release of cardioprotective endothelial-derived nitric oxide prostaglandin E-2 and prostacyclin into the circulation. We investigated whether pGz may be effective as an early preconditioning strategy when applied one hour prior to whole body ischemia reperfusion injury induced by ventricular fibrillation (VF). MAIN METHODS: Twenty anesthetized and paralyzed male swine were randomized to one hour of pGz and conventional mechanical ventilation [PC] or solely conventional mechanical ventilation [Control] prior to VF and resuscitation. After eight minutes of unsupported VF, cardiopulmonary resuscitation was carried out followed by defibrillation. Hemodynamics, electrocardiogram, echocardiogram, regional blood flows, and markers of global myocardial injury were measured. Protein expression of endothelial-derived nitric oxide synthase (eNOS), phosphorylated eNOS (p-eNOS), serine/threonine kinase Akt total (t-Akt), and phosphorylated (p-Akt) were determined by immunoblotting. KEY FINDINGS: All animals had spontaneous return of circulation after cardiopulmonary resuscitation (CPR) and defibrillation. Preconditioned animals had less hemodynamically significant arrhythmias, less myocardial stunning, and greater regional blood flows to the brain, heart, kidneys, and ileum than Controls. Troponin I and creatine phosphokinase values in PC were 65% of the values present in Controls. In addition, preconditioned animals had higher protein expression of cardiac eNOS, p-eNOS, t-Akt, and p-Akt than Controls. SIGNIFICANCE: pGz preconditioning confers early cardioprotection in a model of whole body ischemia reperfusion injury.
Authors:
Jose A Adams; Heng Wu; Jorge A Bassuk; Jaqueline Arias; Arkady Uryash; Vinod Jorapur; Gervasio A Lamas; Paul Kurlansky
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-03-06
Journal Detail:
Title:  Life sciences     Volume:  86     ISSN:  1879-0631     ISO Abbreviation:  Life Sci.     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-04-23     Completed Date:  2010-05-06     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0375521     Medline TA:  Life Sci     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  707-15     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier Inc. All rights reserved.
Affiliation:
Mt Sinai Medical Center, Division Neonatology, Miami Beach, FL 33140, USA. tony@msmc.com
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MeSH Terms
Descriptor/Qualifier:
Acceleration
Animals
Cardiopulmonary Resuscitation / methods
Creatine Kinase / metabolism
Dinoprostone / metabolism
Electric Countershock
Ischemic Preconditioning, Myocardial / methods*
Male
Nitric Oxide / metabolism
Nitric Oxide Synthase Type III / metabolism*
Phosphorylation
Proto-Oncogene Proteins c-akt / metabolism
Random Allocation
Regional Blood Flow
Reperfusion Injury / physiopathology,  prevention & control*
Stress, Mechanical
Swine
Troponin I / metabolism
Ventricular Fibrillation / physiopathology*
Chemical
Reg. No./Substance:
0/Troponin I; 10102-43-9/Nitric Oxide; 363-24-6/Dinoprostone; EC 1.14.13.39/Nitric Oxide Synthase Type III; EC 2.7.11.1/Proto-Oncogene Proteins c-akt; EC 2.7.3.2/Creatine Kinase

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