Document Detail

Perinatal food restriction in rats reduces the content but not concentration of liver extracellular matrix proteins.
MedLine Citation:
PMID:  8487091     Owner:  NLM     Status:  MEDLINE    
Fibrosis of the liver has been reported to be associated with malnutrition, based on qualitative histological and histochemical approaches. The aim of this study was to quantitatively examine the effect of perinatal food-restriction on the expression of extracellular matrix components of the liver, using rats fed 50% of ad libitum intake, determined by the food consumption of the control group on the previous day. Levels of extracellular matrix proteins were measured by dot blot analysis using monospecific antibodies against collagen types I, III and IV, laminin and fibronectin. The body weights of 4, 8 and 13-wk-old malnourished groups were 25, 35 and 48% of the control group body weights, respectively, and the liver weights were 22, 32 and 60% of the controls. The total contents of the extracellular matrix components were significantly reduced to 30, 34 and 58% of the controls, but when expressed per tissue or per milligram protein there were no significant differences. Indirect immunofluorescence revealed no major changes in extracellular matrix localization. The major histological change in the food-restricted rats was fatty infiltration. Our observations suggest that the effect of perinatal food restriction is mainly liver steatosis, and that the liver of food-restricted animals during the perinatal period has the capacity to preserve its main extracellular matrix components.
S Reif; R B Lu; M Tano; V Terranova; C Young; J Fisher; J Petell; E Lebenthal
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of nutrition     Volume:  123     ISSN:  0022-3166     ISO Abbreviation:  J. Nutr.     Publication Date:  1993 May 
Date Detail:
Created Date:  1993-06-07     Completed Date:  1993-06-07     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0404243     Medline TA:  J Nutr     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  811-6     Citation Subset:  IM    
Division of Gastroenterology and Nutrition, Colucci Memorial Liver Research Center, Buffalo, NY.
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MeSH Terms
Animals, Newborn
Body Weight / physiology
Embryonic and Fetal Development / physiology
Extracellular Matrix Proteins / metabolism*
Fluorescent Antibody Technique
Liver / metabolism*,  pathology
Organ Size / physiology
Prenatal Exposure Delayed Effects*
Protein-Energy Malnutrition / metabolism*
Rats, Sprague-Dawley
Reg. No./Substance:
0/Extracellular Matrix Proteins

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