| Perilipin is present in islets of Langerhans and protects against lipotoxicity when overexpressed in the beta-cell line INS-1. | |
| | |
MedLine Citation:
|
PMID: 19299455 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Lipids have been shown to play a dual role in pancreatic beta-cells: a lipid-derived signal appears to be necessary for glucose-stimulated insulin secretion, whereas lipid accumulation causes impaired insulin secretion and apoptosis. The ability of the protein perilipin to regulate lipolysis prompted an investigation of the presence of perilipin in the islets of Langerhans. In this study evidence is presented for perilipin expression in rat, mouse, and human islets of Langerhans as well as the rat clonal beta-cell line INS-1. In rat and mouse islets, perilipin was verified to be present in beta-cells. To examine whether the development of lipotoxicity could be prevented by manipulating the conditions for lipid storage in the beta-cell, INS-1 cells with adenoviral-mediated overexpression of perilipin were exposed to lipotoxic conditions for 72 h. In cells exposed to palmitate, perilipin overexpression caused increased accumulation of triacylglycerols and decreased lipolysis compared with control cells. Whereas glucose-stimulated insulin secretion was retained after palmitate exposure in cells overexpressing perilipin, it was completely abolished in control beta-cells. Thus, overexpression of perilipin appears to confer protection against the development of beta-cell dysfunction after prolonged exposure to palmitate by promoting lipid storage and limiting lipolysis. |
| | |
Authors:
|
Jörgen Borg; Cecilia Klint; Nils Wierup; Kristoffer Ström; Sara Larsson; Frank Sundler; Roberto Lupi; Piero Marchetti; Guoheng Xu; Alan Kimmel; Constantine Londos; Cecilia Holm |
Related Documents
:
|
6326945 - Structure/function relationships of retinal ganglion cells in the cat. 4619525 - Effect of alloxan administration upon channa punctatus (bloch). 1370585 - Pituitary follicular cells secrete an inhibitor of aortic endothelial cell growth: iden... 17052795 - Beta-cell preservation with thiazolidinediones. 8048585 - Role of edrfs in the control of arteriolar diameter during increased metabolism of stri... 23093465 - Chondracanthus tenellus (harvey) hommersand extract protects the human keratinocyte cel... |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Intramural; Research Support, Non-U.S. Gov't Date: 2009-03-19 |
Journal Detail:
|
Title: Endocrinology Volume: 150 ISSN: 1945-7170 ISO Abbreviation: Endocrinology Publication Date: 2009 Jul |
Date Detail:
|
Created Date: 2009-06-24 Completed Date: 2009-07-24 Revised Date: 2013-03-27 |
Medline Journal Info:
|
Nlm Unique ID: 0375040 Medline TA: Endocrinology Country: United States |
Other Details:
|
Languages: eng Pagination: 3049-57 Citation Subset: AIM; IM |
Affiliation:
|
Department of Experimental Medical Science, Lund University, Lund, Sweden. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adult Aged Animals Carrier Proteins Cell Line Female Humans Insulin-Secreting Cells / metabolism* Lipolysis / drug effects Male Mice Middle Aged Palmitates / pharmacology Phosphoproteins / metabolism* Rats Rats, Wistar |
| Chemical | |
Reg. No./Substance:
|
0/Carrier Proteins; 0/Palmitates; 0/Phosphoproteins; 0/perilipin 1 |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Up-regulation of cyclooxygenase-2 expression and prostaglandin E2 production in human endometriotic ...
Next Document: The Acid-labile subunit is required for full effects of exogenous growth hormone on growth and carbo...