Document Detail


Perilipin is present in islets of Langerhans and protects against lipotoxicity when overexpressed in the beta-cell line INS-1.
MedLine Citation:
PMID:  19299455     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Lipids have been shown to play a dual role in pancreatic beta-cells: a lipid-derived signal appears to be necessary for glucose-stimulated insulin secretion, whereas lipid accumulation causes impaired insulin secretion and apoptosis. The ability of the protein perilipin to regulate lipolysis prompted an investigation of the presence of perilipin in the islets of Langerhans. In this study evidence is presented for perilipin expression in rat, mouse, and human islets of Langerhans as well as the rat clonal beta-cell line INS-1. In rat and mouse islets, perilipin was verified to be present in beta-cells. To examine whether the development of lipotoxicity could be prevented by manipulating the conditions for lipid storage in the beta-cell, INS-1 cells with adenoviral-mediated overexpression of perilipin were exposed to lipotoxic conditions for 72 h. In cells exposed to palmitate, perilipin overexpression caused increased accumulation of triacylglycerols and decreased lipolysis compared with control cells. Whereas glucose-stimulated insulin secretion was retained after palmitate exposure in cells overexpressing perilipin, it was completely abolished in control beta-cells. Thus, overexpression of perilipin appears to confer protection against the development of beta-cell dysfunction after prolonged exposure to palmitate by promoting lipid storage and limiting lipolysis.
Authors:
Jörgen Borg; Cecilia Klint; Nils Wierup; Kristoffer Ström; Sara Larsson; Frank Sundler; Roberto Lupi; Piero Marchetti; Guoheng Xu; Alan Kimmel; Constantine Londos; Cecilia Holm
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Intramural; Research Support, Non-U.S. Gov't     Date:  2009-03-19
Journal Detail:
Title:  Endocrinology     Volume:  150     ISSN:  1945-7170     ISO Abbreviation:  Endocrinology     Publication Date:  2009 Jul 
Date Detail:
Created Date:  2009-06-24     Completed Date:  2009-07-24     Revised Date:  2013-03-27    
Medline Journal Info:
Nlm Unique ID:  0375040     Medline TA:  Endocrinology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3049-57     Citation Subset:  AIM; IM    
Affiliation:
Department of Experimental Medical Science, Lund University, Lund, Sweden.
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Animals
Carrier Proteins
Cell Line
Female
Humans
Insulin-Secreting Cells / metabolism*
Lipolysis / drug effects
Male
Mice
Middle Aged
Palmitates / pharmacology
Phosphoproteins / metabolism*
Rats
Rats, Wistar
Chemical
Reg. No./Substance:
0/Carrier Proteins; 0/Palmitates; 0/Phosphoproteins; 0/perilipin 1
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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