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Perilipin, C/EBPalpha, and C/EBPbeta mRNA abundance in longissimus muscle and different adipose tissues of Holstein and Charolais cattle.
MedLine Citation:
PMID:  20416782     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Possible regulators of intramuscular fat deposition were investigated in longissimus muscle (LM) and adipose tissues of 18months old Holstein and Charolais bulls. The mRNA abundance of perilipin and transcription factors C/EBPalpha and C/EBPbeta was analyzed by real-time RT-PCR. Carcass traits and marbling traits were recorded and relationships among adipogenic genes and tissue traits were determined. Charolais cattle were heavier (P<0.001) and had less body fat (P<0.001). Holstein bulls accumulated more fat in the LM (P=0.02), but the number and size of marbling flecks did not differ (P>0.7) between breeds. Perilipin, C/EBPalpha, and C/EBPbeta mRNA abundance was influenced by tissue but not by breed. Relationships between mRNA abundance and marbling traits could not be confirmed, however relationships among adipogenic genes. The transcriptional activity of adipogenic genes in LM suggests that intramuscular adipose tissue is still developing and differentiation still occurs.
Authors:
J X Xu; E Albrecht; T Viergutz; G Nürnberg; R Q Zhao; J Wegner
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2009-4-18
Journal Detail:
Title:  Meat science     Volume:  -     ISSN:  1873-4138     ISO Abbreviation:  -     Publication Date:  2009 Apr 
Date Detail:
Created Date:  2010-4-26     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101160862     Medline TA:  Meat Sci     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Nanjing Agricultural University, Nanjing 210095, PR China.
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