Document Detail

Pericellular-acting proteases in human first trimester decidua.
MedLine Citation:
PMID:  18175789     Owner:  NLM     Status:  MEDLINE    
Proteolysis is essential for decidual development during embryonic implantation, but little is known regarding the expression and functions of membrane-type matrix metalloproteinases (MT-MMPs) and urokinase-type plasminogen activator (uPA) and its receptor uPAR in decidua. Therefore, their protein and mRNA levels were analysed in three first trimester decidual tissues, decidual secretory endometrium (DSE), decidua parietalis (DP) and basalis (DB). Decidua was obtained during first trimester pregnancy termination. uPA, uPAR, and MT1/2/3/5-MMP expression were studied by RT-PCR and immunohistochemistry, and CD56-positive uNK cells and CD68-positive macrophages were quantified in serial sections. The mRNAs and antigens of all proteases and uPAR were detectable in the decidual tissues and extravillous trophoblasts (EVT). mRNA levels of all proteases and uPAR, except MT5-MMP, were elevated in both DB and DP compared to DSE, being significant for MT1-MMP and uPAR in DP. MT2- and MT3-MMP mRNAs in DB were 24- and 10-fold higher than in DSE, and 19- and 7-fold increased compared to DP. At the protein level uPA and uPAR were particularly elevated in DB, while pro-angiogenic MT1- and MT3-MMPs were elevated in both DB and DP compared to DSE. MT2-MMP was prominently present in all conditions. The number of uNK cells was increased in DB and DP versus DSE, while a comparable increase in macrophages did not reach statistical significance. These data are consistent with a differential regulation of pericellular proteases in decidua by pregnancy-induced hormones, immune cells and EVT.
Margreet Plaisier; Pieter Koolwijk; Florian Willems; Frans M Helmerhorst; Victor W M van Hinsbergh
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Publication Detail:
Type:  Journal Article     Date:  2008-01-05
Journal Detail:
Title:  Molecular human reproduction     Volume:  14     ISSN:  1460-2407     ISO Abbreviation:  Mol. Hum. Reprod.     Publication Date:  2008 Jan 
Date Detail:
Created Date:  2008-02-15     Completed Date:  2008-07-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9513710     Medline TA:  Mol Hum Reprod     Country:  England    
Other Details:
Languages:  eng     Pagination:  41-51     Citation Subset:  IM    
Division of Reproductive Medicine, Department of Gynaecology, Leiden University Medical Centre, PO Box 9600, 2300 RC, Leiden, The Netherlands.
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MeSH Terms
Antigens, CD / genetics,  metabolism
Antigens, CD56 / genetics,  metabolism
Antigens, Differentiation, Myelomonocytic / genetics,  metabolism
Decidua / enzymology,  metabolism*
Matrix Metalloproteinase 14 / genetics,  metabolism
Matrix Metalloproteinase 20 / genetics,  metabolism
Peptide Hydrolases / genetics*,  metabolism
Pregnancy Trimester, First
Reverse Transcriptase Polymerase Chain Reaction
Reg. No./Substance:
0/Antigens, CD; 0/Antigens, CD56; 0/Antigens, Differentiation, Myelomonocytic; 0/CD68 antigen, human; EC 3.4.-/Peptide Hydrolases; EC 3.4.24.-/Matrix Metalloproteinase 20; EC Metalloproteinase 14

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