Document Detail


Peri-OVLT E-series prostaglandins and core temperature do not increase after intravenous IL-1beta in pregnant rats.
MedLine Citation:
PMID:  12133861     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Rats have an attenuated febrile response to endogenous pyrogen near the term of pregnancy. Given the fundamental role of E-series prostaglandins (PGEs) in mediating the febrile response to blood-borne endogenous pyrogen, the present experiments were carried out to determine whether PGEs increase in the area surrounding the organum vasculosum laminae terminalis (peri-OVLT) of near-term pregnant (P) rats as in nonpregnant (NP) rats after intravenous (iv) administration of recombinant rat interleukin-1beta (rrIL-1beta). Core temperature was measured by telemetry and peri-OVLT interstitial fluid was sampled in 12 NP and 12 P chronically instrumented, Sprague-Dawley rats by microdialysis for determination of total PGEs by radioimmunoassay. Basal core temperatures were higher in NP compared with P rats (NP 37.9 degrees C +/- 0.5, P 36.9 degrees C +/- 0.4; P < 0.05), but basal peri-OVLT PGEs were similar in both groups (NP 260 +/- 153 pg/ml, P 278 +/- 177 pg/ml; P =not significant). Intravenous administration of rrIL-1beta to NP rats produced a significant increase in core temperature with a latency, magnitude, and duration of 10 min, 0.87 degrees C, and at least 170 min, respectively; peri-OVLT PGEs were increased significantly by 30 min and averaged 270% above basal levels throughout the experiment. In P rats, however, neither core temperature nor peri-OVLT PGEs increased significantly after iv administration of rrIL-1beta. Intravenous administration of vehicle did not significantly alter core temperature or peri-OVLT PGEs in either group of rats. Thus peri-OVLT PGEs do not increase in P rats as they do in NP rats after iv administration of rrIL-1beta. The mechanism of this interesting component of the maternal adaptation to pregnancy, which likely plays a major role in mediating the attenuated febrile response to endogenous pyrogen near the term of pregnancy, warrants further investigation.
Authors:
James E Fewell; Heather L Eliason; Roland N Auer
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of applied physiology (Bethesda, Md. : 1985)     Volume:  93     ISSN:  8750-7587     ISO Abbreviation:  J. Appl. Physiol.     Publication Date:  2002 Aug 
Date Detail:
Created Date:  2002-07-22     Completed Date:  2003-02-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8502536     Medline TA:  J Appl Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  531-6     Citation Subset:  IM    
Affiliation:
Department of Physiology, University of Calgary Health Sciences Centre, Calgary, Alberta, Canada T2N 4N1. fewell@ucalgary.ca
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MeSH Terms
Descriptor/Qualifier:
Animals
Body Temperature / drug effects,  immunology*
Female
Hypothalamus / immunology,  metabolism
Injections, Intravenous
Interleukin-1 / pharmacology*
Microdialysis
Pregnancy
Pregnancy, Animal / immunology*
Prostaglandins E / metabolism*
Pyrogens / pharmacology*
Rats
Rats, Sprague-Dawley
Chemical
Reg. No./Substance:
0/Interleukin-1; 0/Prostaglandins E; 0/Pyrogens

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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