| Perfusion of the isolated rat hind limb. An analysis of the technique. | |
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MedLine Citation:
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PMID: 4079444 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have studied critically the validity, reproducibility, and possible limitations of the rat hind limb perfusion technique under constant flow conditions. Of all the variables studied, bolus size, order of dose administration, perfusion pressure, rat temperature, perfusate temperature, perfusate type, rat age, and rat sex, we found that time is an important limitation in the use of this technique when oxygenated standard physiological salt solutions are used. This limitation may be minimized when a colloid is added to the perfusing medium or eliminated when the perfusion time is no longer than 60 min. |
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Authors:
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A Bomzon; S G Naidu |
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Publication Detail:
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Type: In Vitro; Journal Article |
Journal Detail:
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Title: Journal of pharmacological methods Volume: 14 ISSN: 0160-5402 ISO Abbreviation: J Pharmacol Methods Publication Date: 1985 Dec |
Date Detail:
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Created Date: 1986-02-07 Completed Date: 1986-02-07 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7806596 Medline TA: J Pharmacol Methods Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 285-96 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Age Factors Animals Blood Pressure / drug effects Body Temperature / drug effects Dose-Response Relationship, Drug Female Hindlimb / blood supply* Male Norepinephrine / pharmacology Perfusion / methods* Rats Rats, Inbred Strains Regional Blood Flow Sex Factors Time Factors |
| Chemical | |
Reg. No./Substance:
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51-41-2/Norepinephrine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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