Document Detail


Perfusion cell seeding on large porous PLA/calcium phosphate composite scaffolds in a perfusion bioreactor system under varying perfusion parameters.
MedLine Citation:
PMID:  20872752     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A promising approach to bone tissue engineering lies in the use of perfusion bioreactors where cells are seeded and cultured on scaffolds under conditions of enhanced nutrient supply and removal of metabolic products. Fluid flow alterations can stimulate cell activity, making the engineering of tissue more efficient. Most bioreactor systems are used to culture cells on thin scaffold discs. In clinical use, however, bone substitutes of large dimensions are needed. In this study, MG63 osteoblast-like cells were seeded on large porous PLA/glass scaffolds with a custom developed perfusion bioreactor system. Cells were seeded by oscillating perfusion of cell suspension through the scaffolds. Applicable perfusion parameters for successful cell seeding were determined by varying fluid flow velocity and perfusion cycle number. After perfusion, cell seeding, the cell distribution, and cell seeding efficiency were determined. A fluid flow velocity of 5 mm/s had to be exceeded to achieve a uniform cell distribution throughout the scaffold interior. Cell seeding efficiencies of up to 50% were achieved. Results suggested that perfusion cycle number influenced cell seeding efficiency rather than fluid flow velocities. The cell seeding conducted is a promising basis for further long term cell culture studies in large porous scaffolds.
Authors:
M A Koch; E J Vrij; E Engel; J A Planell; D Lacroix
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-09-24
Journal Detail:
Title:  Journal of biomedical materials research. Part A     Volume:  95     ISSN:  1552-4965     ISO Abbreviation:  J Biomed Mater Res A     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-11-08     Completed Date:  2011-02-22     Revised Date:  2013-04-05    
Medline Journal Info:
Nlm Unique ID:  101234237     Medline TA:  J Biomed Mater Res A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1011-8     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Wiley Periodicals, Inc.
Affiliation:
Institute for Bioengineering of Catalonia (IBEC), Barcelona, Spain.
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MeSH Terms
Descriptor/Qualifier:
Acridine Orange / metabolism
Bioreactors*
Calcium Phosphates / pharmacology*
Cell Culture Techniques / instrumentation*
Cell Line
Cell Survival / drug effects
Ethidium / metabolism
Humans
Lactic Acid / pharmacology*
Osteoblasts / cytology*,  drug effects
Perfusion / methods*
Polymers / pharmacology*
Porosity / drug effects
Rheology / drug effects
Staining and Labeling
Stress, Mechanical
Time Factors
Tissue Scaffolds / chemistry*
Chemical
Reg. No./Substance:
0/Calcium Phosphates; 0/Polymers; 26100-51-6/poly(lactic acid); 3546-21-2/Ethidium; 50-21-5/Lactic Acid; 65-61-2/Acridine Orange; 97Z1WI3NDX/calcium phosphate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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