Document Detail


Performance of serum-free broth media for growth of Renibacterium salmoninarum.
MedLine Citation:
PMID:  9789976     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Growth of Renibacterium salmoninarum was compared in 14 different broth media; 13 serum-free, and 1 that contained newborn calf serum, KDM2+M. Supplementation with 1% v/v R. salmoninarum MCO4M metabolite was evaluated for 6 of the media that do not utilize it as part of their ingredients. Viable cells were enumerated on Days 10, 20, and 30 post inoculation to evaluate performance. The experiment was repeated 3 times using high, low, and medium (trials 1 to 3, respectively) cell concentrations as inoculum. In general there was no optimal medium and all performed well. The choice of which to employ depends on the ease of preparation and presence of certain ingredients that might affect subsequent assays. In trials 2 and 3, the pH was estimated using test papers at the same time as cells were counted. Maximum pH increase occurred with KDM2+M and those media containing charcoal. For most media, a simple pH determination could be used as a means to check that growth has occurred in a culture, particularly if charcoal was added directly to the media and a visual inspection could not be made to detect growth.
Authors:
C E Starliper; W B Schill; J Mathias
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Diseases of aquatic organisms     Volume:  34     ISSN:  0177-5103     ISO Abbreviation:  Dis. Aquat. Org.     Publication Date:  1998 Sep 
Date Detail:
Created Date:  1998-12-21     Completed Date:  1998-12-21     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8807037     Medline TA:  Dis Aquat Organ     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  21-6     Citation Subset:  IM    
Affiliation:
National Fish Health Research Laboratory, Biological Resources Division, Kearneysville, West Virginia 25430, USA. cliff_starliper@usgs.gov
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MeSH Terms
Descriptor/Qualifier:
Analysis of Variance
Animals
Colony Count, Microbial
Culture Media, Serum-Free*
Evaluation Studies as Topic
Fish Diseases / microbiology
Gram-Positive Bacteria / growth & development*
Gram-Positive Bacterial Infections / microbiology,  veterinary
Hydrogen-Ion Concentration
Kidney Diseases / microbiology,  veterinary
Salmonidae
Chemical
Reg. No./Substance:
0/Culture Media, Serum-Free

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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