| Performance of a new chromogenic plating medium for the isolation of Listeria monocytogenes from marine environments. | |
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MedLine Citation:
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PMID: 15644105 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIMS: This study investigated the performance of a new chromogenic plating medium for the detection of Listeria monocytogenes from naturally contaminated samples obtained from marine environments in Morocco in comparison with the conventional plating media PALCAM and Oxford. METHODS: A total of 479 marine samples (sea water, sediment and mussels) were collected from 16 littoral sites in the region of Agadir (western centre of Morocco). They were examined for the presence of L. monocytogenes using a slight modification of the standardized French method (AFNOR V 08-055) for the detection of L. monocytogenes from food and three different isolation media: PALCAM, Oxford and a new chromogenic plating medium. RESULTS AND SIGNIFICANCE OF THE STUDY: The Oxford and the new chromogenic plating media were found relatively more efficient than the PALCAM medium for the isolation of L. monocytogenes (chi-square test, P < 0.05) from marine samples. However, the new chromogenic plating medium was significantly more selective for L. monocytogenes (P < 0.005) than the two other isolation media as 87.5% of the suspect colonies on this medium were indeed confirmed through identification of the isolates vs 12.7% for Oxford and only 3.8% for the PALCAM medium. |
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Authors:
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A El Marrakchi; N Boum'handi; A Hamama |
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Publication Detail:
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Type: Comparative Study; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Letters in applied microbiology Volume: 40 ISSN: 0266-8254 ISO Abbreviation: Lett. Appl. Microbiol. Publication Date: 2005 |
Date Detail:
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Created Date: 2005-01-12 Completed Date: 2005-03-03 Revised Date: 2013-05-20 |
Medline Journal Info:
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Nlm Unique ID: 8510094 Medline TA: Lett Appl Microbiol Country: England |
Other Details:
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Languages: eng Pagination: 87-91 Citation Subset: IM |
Affiliation:
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Institut Agronomique et Vétérinaire Hassan II, Rabat-Instituts, Rabat, Morocco. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Agar Animals Bacteriological Techniques Bivalvia / microbiology* Chromogenic Compounds / metabolism* Culture Media* Geologic Sediments / microbiology Listeria monocytogenes / growth & development, isolation & purification* Morocco Seawater / microbiology* Shellfish / microbiology* |
| Chemical | |
Reg. No./Substance:
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0/Chromogenic Compounds; 0/Culture Media; 9002-18-0/Agar |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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