Document Detail


Performance of a matrix-assisted laser desorption ionization-time-of-flight mass spectrometry system for the identification of bacterial isolates in the clinical routine laboratory.
MedLine Citation:
PMID:  19894408     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Matrix-Assisted Laser Desorption Ionization-Time-of-Flight Mass Spectrometry (MALDI-TOF MS) has emerged as a new tool for the fast and reliable identification of microorganisms. We evaluated the performance of a MALDI-TOF MS-system for the identification of various clinical isolates in the routine microbiology setting. MATERIAL AND METHODS: For the evaluation study a set of 1116 bacterial isolates were collected in the routine microbiology laboratory. Additonally 108 isolates of strain culture collections (ATTC, DSMZ) were utilized. Identification of the bacterial isolates was perfomed with a Microflex LT mass spectrometer in combination with the MALDI-Biotyper 2.0 software (Bruker Daltonik GmbH, Bremen, Germany). The results of the MALDI-TOF MS were compared to phenotypic bacterial identification systems used in our routine laboratory. Discrepancies were resolved by 16 S rDNA-sequencing. RESULTS: Of the 108 reference strains tested, 101 (93.5%) were correctly identified to species level. Overall, 1062 (95.2%) of the 1116 strains collected in the routine laboratory were correctly identified with the MALDI-Biotyper. Accuracy for the identification of Enterobacteriaceae, non-fermenting gram-negative rods, staphylococci, enterococci and streptococci with the MALDI-Biotyper was 95.5%, 79.7%, 99.5%, 100% and 93.7%, respectively. Results were available in 12 minutes for direct smear and in 20 min with an extraction method. CONCLUSIONS: The MALDI-TOF method proves to be a fast and reliable method for the identification of the most important bacterial isolates in the clinical laboratory.
Authors:
Ulrich Eigner; Martin Holfelder; Klaus Oberdorfer; Ulrike Betz-Wild; Daniela Bertsch; Anne-Marie Fahr
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Clinical laboratory     Volume:  55     ISSN:  1433-6510     ISO Abbreviation:  Clin. Lab.     Publication Date:  2009  
Date Detail:
Created Date:  2009-11-09     Completed Date:  2010-01-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9705611     Medline TA:  Clin Lab     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  289-96     Citation Subset:  IM    
Affiliation:
Department of Microbiology, Laboratory Limbach, Heidelberg, Germany. ulrich.eigner@labor-limbach.de
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MeSH Terms
Descriptor/Qualifier:
Bacteria / isolation & purification*
DNA Primers
Diagnostic Tests, Routine / methods*
Gram-Negative Bacteria / classification,  genetics,  isolation & purification
Gram-Positive Bacteria / classification,  genetics,  isolation & purification
Humans
Phenotype
Polymerase Chain Reaction
Reproducibility of Results
Sensitivity and Specificity
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
Chemical
Reg. No./Substance:
0/DNA Primers

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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